Puccinia triticina effector protein Pt_21 interacts with wheat thaumatin-like protein TaTLP1 to inhibit its antifungal activity and suppress wheat apoplast immunity  

作　　者：Fei Wang Songsong Shen Zhongchi Cui Shitao Yuan Ping Qu Hui Jia Linshuo Meng Xiaoyu Hao Daqun Liu Lisong Ma Haiyan Wang 

机构地区：[1]College of Plant Protection,Hebei Agricultural University/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province,Baoding 071001,Hebei,China [2]Center of Agriculture Through Science and Education,Hebei Agricultural University,Baoding 071001,Hebei,China [3]The State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071001,Hebei,China

出　　处：《The Crop Journal》2023年第5期1431-1440,共10页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (32172384 and 31501623);the Natural Science Foundation of Hebei (C2020204028);the Key Research and Development Project of Hebei Province (20326505D);the “Hundred Talents Program” for the Introduction of High-level Overseas Talents in Hebei Province (E2020100004)。

摘　　要：Puccinia triticina(Pt), as the causal agent of wheat leaf rust, employs a plethora of effector proteins to modulate wheat immunity for successful colonization. Understanding the molecular mechanisms underlying Pt effector-mediated wheat susceptibility remains largely unexplored. In this study, an effector Pt_21 was identified to interact with the apoplast-localized wheat thaumatin-like protein TaTLP1 using a yeast two-hybrid assay and the Pt_21-TaTLP1 interaction was characterized. The interaction between Pt_21 and TaTLP1 was validated by in vivo co-immunoprecipitation assay. A TaTLP1 variant,TaTLP1C71A, that was identified by the site-directed mutagenesis failed to interact with Pt_21. Pt_21was able to suppress Bax-mediated cell death in leaves of Nicotiana benthamiana and inhibit TaTLP1-mediated antifungal activity. Furthermore, infiltration of recombinant protein Pt_21 into leaves of transgenic wheat line overexpressing TaTLP1 enhanced the disease development of leaf rust compared to that in wild-type leaves. These findings demonstrate that Pt_21 suppresses host defense response by directly targeting wheat TaTLP1 and inhibiting its antifungal activity, which broadens our understanding of the molecular mechanisms underlying Pt effector-mediated susceptibility in wheat.

关 键 词：WHEAT Puccinia triticina EFFECTOR Thaumatin-like protein Anitifungal activity 

分 类 号：S512.1[农业科学—作物学]