miR-125b-2-3p表达对鼻咽癌细胞HNE1增殖和凋亡的影响  

Effect of miR-125b-2-3p expression on proliferation and apoptosis of nasopharyngeal carcinoma cells HNE1

作　　者：陶露 汤迎凯 韦卓利 项平[1] TAO Lu;TANG Yingkai;WEI Zhuoli;XIANG Ping(Central Laboratory of First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,China;Department of Human Anatomy,Bengbu Medical College;Anhui Key Laboratory of Tissue Transplantation,Bengbu Medical College)

机构地区：[1]蚌埠医学院第一附属医院中心实验室,蚌埠233000 [2]蚌埠医学院人体解剖学教研室 [3]蚌埠医学院组织移植安徽省重点实验室

出　　处：《山西医科大学学报》2023年第9期1192-1199,共8页Journal of Shanxi Medical University

基　　金：蚌埠医学院自然科学重点项目(2021byzd048);安徽省高等学校科学研究项目(2022AH051481)。

摘　　要：目的探讨miR-125b-2-3p表达对鼻咽癌细胞HNE1增殖和凋亡的影响。方法荧光定量PCR检测miR-125b-2-3p在人永生化鼻咽上皮细胞NP69和鼻咽癌细胞中的表达。荧光定量PCR检测上调和下调miR-125b-2-3p表达转染效率。实验分为control组(未处理)、mimics NC组(Lip 2000+mimics NC)、miR-125b-2-3p mimics组(Lip 2000+miR-125b-2-3p mimics)、inhibitor NC组(Lip 2000+inhibitor NC)和miR-125b-2-3p inhibitor组(Lip 2000+miR-125b-2-3p inhibitor)。CCK-8实验、细胞集落形成实验和活细胞/死细胞双染色实验检测细胞增殖的情况;流式细胞术检测细胞凋亡情况;DAPI细胞核染色检测细胞核的形态变化;线粒体膜电位检测细胞早期凋亡情况。结果与人永生化鼻咽上皮细胞NP69相比,鼻咽癌细胞中miR-125b-2-3p的表达较高(P<0.05)。与control组和mimics NC组相比,miR-125b-2-3p mimics组miR-125b-2-3p表达上调(P<0.01);与control组和inhibitor NC组相比,miR-125b-2-3p inhibitor组miR-125b-2-3p表达下调(P<0.01)。与mimics NC组相比,miR-125b-2-3p mimics组细胞增殖能力较强(P<0.01),细胞凋亡能力较弱(P<0.01),线粒体膜电位较高(P<0.01);相较于inhibitor NC组,miR-125b-2-3p inhibitor组细胞增殖能力被抑制(P<0.01),细胞凋亡能力较强(P<0.01),核固缩核碎裂较多,线粒体膜电位较低(P<0.05)。结论下调miR-125b-2-3p能抑制鼻咽癌细胞HNE1的增殖,促进细胞的凋亡。Objective To investigate the effects of miR-125b-2-3p expression on the proliferation and the apoptosis of nasopharyngeal carcinoma cells HNE1.Methods The expression of miR-125b-2-3p in nasopharyngeal carcinoma cells HNE1 and human immortarized nasopharyngeal epithelial cells NP69 was detected by fluorescence quantitative PCR.Fluorescence quantitative PCR was used to detect miR-125b-2-3p expression.The experiment was divided into control group(no treatment),mimics NC group(Lip 2000+mimics NC),miR-125b-2-3p mimics group(Lip 2000+miR-125b-2-3p mimics)and inhibitor NC group(Lip 2000+inhibitor NC)and miR-125b-2-3p inhibitor group(Lip 2000+miR-125b-2-3p inhibitor).Cell proliferation was detected by CCK-8 assay,cell colony formation assay and live/dead cell double staining assay.Cell apoptosis was detected by flow cytometry.DAPI nuclear staining was used to detect the morphological changes of the nuclei.Early cell apoptosis was detected by mitochondrial membrane potential kit.Results The expression of miR-125b-2-3p in nasopharyngeal carcinoma cells HNE1 was higher than that in human immortarized nasopharyngeal epithelial cells NP69(P<0.05).Compared with control group and mimics NC group,miR-125b-2-3p expression was upregulated in miR-125b-2-3p mimics group(P<0.01).Compared with control group and inhibitor NC group,miR-125b-2-3p was downregulated in miR-125b-2-3p inhibitor group(P<0.01).Compared with mimics NC group,the cell proliferation ability was increased in miR-125b-2-3p mimics group(P<0.01),the apoptosis ability was decreased(P<0.01),and the mitochondrial membrane potential was increased(P<0.01).Compared with inhibitor NC group,the cell proliferation was inhibited in miR-125b-2-3p inhibitor group(P<0.01),the apoptosis ability was increased(P<0.01),nuclear pycnosis and fragmentation were increased,and the mitochondrial membrane potential was decreased(P<0.05).Conclusion Downregulation of miR-125b-2-3p can inhibit the proliferation and promote the apoptosis of nasopharyngeal carcinoma cells HNE1.

关 键 词：miR-125b-2-3p 鼻咽癌 细胞增殖 细胞凋亡 HNE1 

分 类 号：R739.6[医药卫生—肿瘤]