一种氧化型染发剂的致突变作用研究  

Study on the Mutagenic Effects of an Oxidative Hair Dye

作　　者：罗吉 李冰 赵少浩 于志强 刘汉伟 黄昕 LUO Ji;LI Bing;ZHAO Shao-Hao;YU Zhi-Qiang;LIU Han-Wei;HUANG Xin(Ningbo Customs District,Ningbo 315000;Ningbo Joysun Product Testing Co.,Ltd.,Ningbo 315000)

机构地区：[1]宁波海关,宁波315000 [2]宁波中盛产品检测有限公司,宁波315000

出　　处：《中国口岸科学技术》2023年第9期76-81,共6页China Port Science and Technology

基　　金：海关总署科研项目(2021HK227)。

摘　　要：本研究采用鼠伤寒沙门菌回复突变试验(Ames试验)、体内哺乳动物细胞微核试验和体外哺乳动物细胞染色体畸变试验评估一种氧化型染发剂的致突变性。结果表明,Ames试验在加S9的情况下,菌株TA98在12.5μL/皿、25.0μL/皿、50.0μL/皿、100.0μL/皿剂量组中的回变菌落数是溶剂对照组回变菌落数的2~3倍,结果显示为阳性。微核试验为阴性,未见体内染色体畸变作用。体外哺乳动物细胞染色体畸变试验结果为阴性,未见致突变性。该试验结果为后续对染发剂的致突变作用研究提供参考。This study used the salmonella typhimurium reversion mutation test(Ames test), in vivo mammalian cell micronucleus test, and in vitro mammalian cell chromosomal aberration test to evaluate the mutagenicity of an oxidative hair dye. In the Ames test with S9, the number of revertant colonies of strain TA98 in 12.5 μL per dish, 25.0 μL per dish, 50.0 μL per dish and 100.0 μL per dish dose groups were 2-3 times higher than that in solvent control group, showing a positive result. The micronucleus test was negative,with no observed in vivo chromosomal aberration effect. The in vitro mammalian cell chromosomal aberration test also showed negative results, with no observed mutagenicity. The results of this experiment provide a reference for subsequent research on the mutagenicity of hair dyes.

关 键 词：染发剂 AMES试验 微核试验 染色体畸变 

分 类 号：R99[医药卫生—毒理学] TQ658.34[医药卫生—药学]