预知子总皂苷大孔树脂分离纯化工艺优化及抗氧化活性研究  

Optimization of Purification Process of Total Saponins from Akebiae Fructus by Macroporous Resin and Research on Its Antioxidant Activity

作　　者：杨晨 武正华 石文清 吴江平 范国荣 YANG Chen;WU Zhenghua;SHI Wenqing;WU Jiangping;FAN Guorong(College of Pharmacy,Anhui University of Chinese Medicine,Hefei,Anhui,China 230012;The General Hospital Affiliated to Shanghai Jiao Tong University,Shanghai,China 200940;Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine,Shanghai,China 200434)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]上海交通大学附属第一人民医院,上海200940 [3]同济大学附属上海市第四人民医院,上海200434

出　　处：《中国药业》2023年第19期57-62,共6页China Pharmaceuticals

基　　金：上海市卫生健康委员会临床重点专科项目[shslczdzk06501]。

摘　　要：目的 优化大孔树脂分离纯化预知子总皂苷的工艺,并对其体外抗氧化活性进行研究。方法 通过比较不同树脂对预知子提取物的吸附率、解吸附率、解吸附效果,选择适当的大孔树脂对预知子进行分离纯化;以总皂苷含量为指标,通过单因素试验考察上样条件;采用Box-Behnken响应面试验优化预知子总皂苷洗脱工艺。通过检测预知子总皂苷对2,2-二苯基-1-苦基肼(DPPH)、2,2-联氮-二(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基的清除能力及总抗氧化能力,评价其体外抗氧化活性。结果 上样条件为S-8型大孔树脂,上样液质量浓度为200 mg/mL,上样量与树脂体积比为2∶1(V/V),pH为2.5,流速为1.0 mL/min;洗脱条件为9 BV 60%乙醇溶液洗脱,流速为1.0 mL/min。预知子总皂苷对DPPH和ABTS自由基清除率的半数抑制浓度分别为0.15,1.54 mg/mL;总抗氧化能力为4.86μmol/mL。结论 S-8型大孔树脂能很好地分离纯化预知子总皂苷,优化的上样及洗脱工艺可为该药的开发提供理论依据。预知子总皂苷具有一定抗氧化活性。Objective To optimize the separation and purification process of total saponins from Akebiae Fructus by macroporous resin,and to study their in vitro antioxidant activity.Methods Appropriate macroporous resins were selected for separation and purification of Akebiae Fructus by comparing the adsorption rate,desorption rate,and desorption effect of different resins on the Akebiae Fructus extracts.Taking the total saponins content as the index,the sample loading conditions were determined by the single factor test,and the elution process of total saponins from Akebiae Fructus was optimized by the Box-Behnken response surface design.The scavenging capacity of DPPH and ABTS free radicals and the total antioxidant capacity of saponins from Akebiae Fructus were determined to evaluate their antioxidant activities in vitro.Results The sample loading conditions were determined as follows:S-8 macroporous resin was used,the mass concentration of the sample solution was 200 mg/mL,the ratio of sample volume to resin volume was 2∶1(V/V),the pH was 2.5,and the flow rate was 1.0 mL/min.The sample was eluted with 9 BV 60% ethanol solution at a flow rate of 1.0 mL/min.The IC50values of total saponins of Akebiae Fructus on DPPH and ABTS radical scavenging were 0.15 mg/mL and 1.54 mg/mL,respectively.The total antioxidant capacity was 4.86 μmol/mL.Conclusion S-8 macroporous resin can effectively separate and purify the total saponins of Akebiae Fructus,and the optimal sample loading and elution process can provide a theoretical basis for the development of this herb.The total saponins of Akebiae Fructus have certain antioxidant activity.

关 键 词：预知子 大孔树脂 总皂苷 分离纯化 抗氧化活性 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]