基于单细胞转录组测序的肠型胃癌上皮细胞特征分析  

Characterization of Epithelial Cells in Intestinal Gastric Cancer Based on Single-cell Transcriptome Sequencing

作　　者：邓高桦 倪培萍 郝涛 梁思成 余泽辉 吕沐瀚 DENG Gaohua;NI Peiping;HAO Tao;LIANG Sicheng;YU Zehui;LU Muhan(Department of Gastroenterology,the Affiliated Hospital of Southwest Medical University,Luzhou,646000)

机构地区：[1]西南医科大学附属医院消化内科,泸州646000

出　　处：《基因组学与应用生物学》2023年第6期655-664,共10页Genomics and Applied Biology

基　　金：国家自然科学基金项目(81972296);四川省科技创新基地(平台)和人才项目(2021JDTD0003)共同资助。

摘　　要：为探索肠型胃癌(intestinal gastric cancer,IGC)的细胞构成,分析其上皮细胞特征的差异,本研究根据公共数据库中肠型胃癌的单细胞转录组测序(single-cell transcriptome sequencing,scRNA-seq)数据,通过质控、降维、聚类和细胞注释后,使用差异基因表达分析、富集分析、轨迹分析和细胞通信分析等方法,探索IGC上皮细胞在构成和分布、功能和状态,以及与微环境中细胞相互作用等方面的特征。结果显示,不同患者在上皮细胞类型比例分布上呈现出异质性。其中,肠细胞在肿瘤组织中的比例明显增多,并有着更高的拷贝数变异和致癌通路活性。肠细胞亚群在功能和状态上也有着差异。在细胞通信中,肿瘤上皮细胞与微环境中不同细胞类型之间有着活跃的细胞间相互作用。本研究揭示了IGC的细胞类型构成以及上皮细胞特征的异质性,为新靶点的开发提供了初步证据和理论基础。In order to explore the cellular composition and to analyze the differences in characterization of epithelial cells in intestinal gastric cancer(IGC),single-cell transcriptome sequencing analysis(scRNA-seq)was used in this study.After dimensionality reduc-tion,clustering and cell annotation,differential gene expression analysis,enrichment analysis,trajectory analysis and cellular commu-nication analysis were used to explore the characteristics of IGC epithelial cells in terms of composition and distribution,function and status,and cellular interactions.The results showed that different patients had heterogeneity in the proportional distribution of epithelial cell types.Among them,the proportion of intestinal cells were significantly increased in tumor tissues,and intestinal cells had a higher copy number variation and oncogenic pathway activity.Intestinal cell subpopulations also differed in function and status.In cellular communication,there were active intercellular interactions between epithelial cells and different cell types.The study reveals the cell type composition of IGC and the heterogeneity of epithelial cell characteristics,provides preliminary evidence and theoretical basis for the development of new targets.

关 键 词：肠型胃癌 单细胞转录组测序 肿瘤异质性 上皮细胞 

分 类 号：R735.2[医药卫生—肿瘤]