Schisandrin B Protects against Ischemic Brain Damage by Regulating PI3K/AKT Signaling in Rats  

作　　者：HONG Quan-long DING Yi-hang CHEN Jing-yi SHI Song-sheng LIANG Ri-sheng TU Xian-kun 

机构地区：[1]Department of Neurology,The First Hospital of Quanzhou Affiliated to Fujian Medical University,Quanzhou,Fujian Province,362000,China [2]Department of Neurosurgery,Fujian Medical University Union Hospital,Fuzhou,350001,China

出　　处：《Chinese Journal of Integrative Medicine》2023年第10期885-894,共10页中国结合医学杂志（英文版）

基　　金：the Natural Science Foundation of Fujian Province of China(No.2022J01245);the Quanzhou City Science&Technology Program of China(No.2020N020s);the Excellent Young Scholars Cultivation Project of Fujian Medical University Union Hospital(No.2022XH040)。

摘　　要：Objective To explore the effect and mechanism of schisandrin B(Sch B)in the treatment of cerebral ischemia in rats.Methods The cerebral ischemia models were induced by middle cerebral artery occlusion(MCAO)and reperfusion.Sprague-Dawley rats were divided into 6 groups using a random number table,including sham,MCAO,MCAO+Sch B(50 mg/kg),MCAO+Sch B(100 mg/kg),MCAO+Sch B(100 mg/kg)+LY294002,and MCAO+Sch B(100 mg/kg)+wortmannin groups.The effects of Sch B on pathological indicators,including neurological deficit scores,cerebral infarct volume,and brain edema,were subsequently studied.Tissue apoptosis was identified by terminal transferase-mediated dUTP nick end-labeling(TUNEL)staining.The protein expressions involved in apoptosis,inflammation response and oxidative stress were examined by immunofluorescent staining,biochemical analysis and Western blot analysis,respectively.The effect of Sch B on phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling was also explored.Results Sch B treatment decreased neurological deficit scores,cerebral water content,and infarct volume in MCAO rats(P<0.05 or P<0.01).Neuronal nuclei and TUNEL staining indicated that Sch B also reduced apoptosis in brain tissues,as well as the Bax/Bcl-2 ratio and caspase-3 expression(P<0.01).Sch B regulated the production of myeloperoxidase,malondialdehyde,nitric oxide and superoxide dismutase,as well as the release of cytokine interleukin(IL)-1βand IL-18,in MCAO rats(P<0.05 or P<0.01).Sch B promoted the phosphorylation of PI3K and AKT.Blocking the PI3K/AKT signaling pathway with LY294002 or wortmannin reduced the protective effect of Sch B against cerebral ischemia(P<0.05 or P<0.01).Conclusions Sch B reduced apoptosis,inflammatory response,and oxidative stress of MCAO rats by modulating the PI3K/AKT pathway.Sch B had a potential for treating cerebral ischemia.

关 键 词：cerebral ischemia inflammation NEUROPROTECTION PI3K/AKT signaling schisandrin B Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]