术前rhAd-p53注射液联合放射治疗对局部晚期子宫颈癌的效果及对病灶细胞增殖/凋亡相关蛋白的影响  

Effect of Preoperative rhAd-p53 Injection Combined with Radiotherapy for Locally Advanced Cervical Cancer and Its Influence on Proliferation/Apoptosis-related Proteins of Lesion Cells

作　　者：林布雷 柯瑞全 LIN Bulei;KE Ruiquan(The First Affiliated Hospital of Xiamen University,Xiamen 361003,China;不详)

机构地区：[1]厦门大学附属第一医院,福建厦门361003

出　　处：《中外医学研究》2023年第26期153-157,共5页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的:探讨术前重组人p53腺病毒(rhAd-p53)注射液联合放射治疗对局部晚期子宫颈癌的效果及对病灶细胞增殖/凋亡相关蛋白的影响。方法:选取2018年7月—2020年6月厦门大学附属第一医院肿瘤放疗科收治的80例局部晚期子宫颈癌患者为研究对象,根据治疗方案不同将80例患者分为对照组和观察组,各40例。对照组实施单纯放射治疗,观察组实施术前rhAd-p53注射液联合放射治疗。比较两组近期疗效、不良反应、治疗前后病灶细胞增殖/凋亡相关蛋白[血管内皮生长因子(VEGF)、p53、半胱天冬氨酸酶-3(Caspase-3)及B淋巴细胞瘤-2基因(Bcl-2)]及血清肿瘤标志物[癌胚抗原(CEA)、糖类抗原125(CA125)及鳞状细胞癌抗原(SCC-Ag)]水平变化。结果:观察组客观缓解率为87.5%,高于对照组的62.5%,差异有统计学意义(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。观察组治疗后VEGF及Bal-2阳性表达率低于对照组,p53及Caspase-3阳性表达率高于对照组,差异有统计学意义(P<0.05)。治疗后,两组CEA、CA125及SCC-Ag较治疗前降低,且观察组低于对照组,差异有统计学意义(P<0.05)。结论:术前rhAd-p53注射液联合放射治疗局部晚期子宫颈癌能上调细胞凋亡蛋白,下调抑制细胞增殖蛋白,从而提高近期疗效,降低血清肿瘤标志物水平,且不会增加治疗期间不良反应。Objective:To investigate the effect of preoperative Recombinant Human Adenovirus p53(rhAd-p53)Injection combined with radiotherapy on locally advanced cervical cancer and its influence on cell proliferation/apoptosis-related proteins of lesion cells.Method:A total of 80 patients with locally advanced cervical cancer admitted to the Radiotherapy Department of the First Affiliated Hospital of Xiamen University from July 2018 to June 2020 were selected as the research objects.According to different treatment regimens,they were divided into control group and observation group,40 cases in each group.The control group was given radiotherapy alone,and the observation group was given preoperative rhAd-p53 Injection combined radiotherapy.The short-term efficacy,adverse reactions,changes of cell proliferation/apoptosis-related proteins of lesion cells[vascular endothelial growth factor(VEGF),p53,Caspase-3 and B-cell lymphoma-2 gene(Bcl-2)]and serum tumor markers[carcinoembryonic antigen(CEA),carbohydrate antigen 125(CA125)and squamous cell carcinoma antigen(SCC-Ag)]before and after treatment were compared between two groups.Result:The objective remission rate of the observation group was 87.5%,which was higher than 62.5%of the control group,and the difference was statistically significant(P<0.05).There were no significant differences in adverse reactions rates between two groups(P>0.05).The positive expression rates of VEGF and Bal-2 in the observation group after treatment were lower than those in the control group,the positive expression rates of p53 and Caspase-3 were higher than those in control group,the differences were statistically significant(P<0.05).After treatment,CEA,CA125 and SCC-Ag in two groups were lower than those before treatment,and those in the observation group were lower than those in the control group,the differences were statistically significant(P<0.05).Conclusion:Preoperative rhAd-p53 Injection combined with radiotherapy in the treatment of locally advanced cervical cancer can up-regulate apoptoti

关 键 词：基因治疗 放射治疗 子宫颈癌 疗效 凋亡蛋白 

分 类 号：R737.33[医药卫生—肿瘤]