茶多酚对马氏珠母贝外套膜组织和细胞培养的影响  

Effects of tea polyphenol on tissue and cell culture of Pinctada martensii mantle

作　　者：方伟 丁瑞霞 陈明强 王雨[1,2,3] 赵旺 温为庚[1,2,3] 马振华 FANG Wei;DING Ruixia;CHEN Mingqiang;WANG Yu;ZHAO Wang;WEN Weigeng;MA Zhenhua(Sanya Tropical Fisheries Research Institute/Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province,Sanya 572000,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Tropical Fisheries Research and Development Center,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Sanya 572018,China)

机构地区：[1]三亚热带水产研究院/海南省深远海渔业资源高效利用与加工重点实验室,海南三亚572000 [2]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [3]中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚572018

出　　处：《南方水产科学》2023年第5期58-65,共8页South China Fisheries Science

基　　金：海南省科技计划三亚崖州湾科技城联合项目(320LH009);中国水产科学研究院基本科研业务费专项资金(2020TD55)。

摘　　要：为改善马氏珠母贝(Pinctada martensii)外套膜组织和细胞体外培养的效果,探究了添加不同浓度的天然提取物茶多酚对外套膜组织和细胞培养液的优化效果。结果显示,当茶多酚的添加量为0.5%~2.0%(体积分数)时,马氏珠母贝外套膜细胞的生物活性显著高于对照组(P<0.05);当添加量为1.0%时,相对细胞生物活性最高。5组完全培养液(0%、0.5%、1.0%、1.5%、2.0%组)均能较好地促进细胞的生长增殖,且细胞的生长曲线均呈“S”型;当培养液中茶多酚的添加量为1.0%时,外套膜细胞增殖效果最优。研究表明,马氏珠母贝外套膜组织和细胞在最适培养液中均能大量增殖游离细胞,且正常分泌珍珠质,其中培养液中的钙离子(Ca^(2+))浓度随着组织和细胞增殖过程呈先减后增的趋势,但始终低于初始浓度。研究结果进一步优化了马氏珠母贝外套膜组织和细胞培养液,为推动马氏珠母贝无核珍珠的培育提供了理论基础。To improve the effects of tea polyphenols on tissue and cell culture of Pinctada martensii mantle,we explored the optimization effect of adding different concentrations of natural extract tea polyphenols on the mantle tissue and cell culture medium.The results show that when the added amount of tea polyphenols was 0.5%–2.0%,the biological activity of P.martensii mantle cells was significantly higher than that of the control group,and the best added amount was 1.0%.The five groups of completely culture medium(0%,0.5%,1.0%,1.5%and 2.0%)all promoted the growth and proliferation of cells,and the growth curves of the cells were all in an"S"shape.When the addition of tea polyphenols was 1%,the proliferation effect of the mantle cells was the best.The mantle tissue and cells proliferated a large number of free cells and secreted nacre normally in the optimal culture medium.The mass concentration of Ca^(2+) in the culture medium decreased first and then increased along with the pro-cess of tissue and cell proliferation,but was always lower than the initial concentration.The study further optimized the P.martensii mantle tissue and cell culture medium,which provides a theoretical basis for promoting the cultivation of P.martensii nuclear pearls.

关 键 词：马氏珠母贝 茶多酚 外套膜 细胞培养 细胞活性 细胞增殖 

分 类 号：S966.2[农业科学—水产养殖]