DACT3在卵巢癌中的表达及其意义  

Expression and significance of DACT3 in ovarian cancer

作　　者：徐慧 王璇 韩勇 石宇[3] XU Hui;WANG Xuan;HAN Yong;SHI Yu(Department of Obsetetrics and Gynecology,Huai’an Second People’s Hospital,Jiangsu 223002;Medical College of Nantong University;Department of Radiation Oncology,Affiliated Hospital of Nantong University;Cancer Center of the First People’s Hospital affiliated to the Shanghai Jiao Tong University)

机构地区：[1]淮安市第二人民医院妇产科,江苏223002 [2]南通大学医学院 [3]南通大学附属医院肿瘤放疗科 [4]上海交通大学附属第一人民医院肿瘤中心

出　　处：《交通医学》2023年第4期341-344,共4页Medical Journal of Communications

基　　金：南通市科技项目(JC2021148,JC2021150)。

摘　　要：目的:探究β-环连蛋白抑制基因3(dishevelled-binding antagonist of beta-catenin 3,DACT3)在卵巢癌中的表达及其预后意义。方法:从TCGA及GTEx数据库中收集卵巢癌组织基因测序数据,分析DACT3表达与卵巢癌患者总生存期(overall survival,OS)及无进展生存期(progression-free survival,PFS)的相关性。选择60例手术切除的浆液性卵巢癌及正常卵巢组织标本,采用免疫组化检测组织中DACT3表达,分析DACT3表达与患者临床病理特征及OS的相关性,采用单因素和多因素COX回归分析影响卵巢癌预后的危险因素。采用细胞划痕实验观察siRNA转染对人卵巢癌腺癌细胞株SKOV3迁移能力的影响。结果:TCGA及GTEx数据库数据显示,DACT3高表达患者的OS和PFS较短(P<0.05),COX回归分析显示,DACT3表达是影响卵巢癌预后的独立危险因素(P=0.00051)。对60例临床卵巢癌患者分析显示,DACT3表达与肿瘤分化程度及FIGO分期相关(P<0.05),DACT3高表达患者OS较短。免疫组化显示,卵巢癌组织中DACT3高表达,高级别卵巢癌组织中DACT3表达较低级别更强。干扰DACT3表达后SKOV3细胞迁移能力显著减弱。结论:DACT3在卵巢癌中高表达,是影响卵巢癌预后的独立危险因素,有可能作为卵巢癌患者的预后指标和分子治疗的靶标。Objective:To investigate the expression and prognostic significance of dishevelled-binding antagonist of beta-catenin 3(DACT3)in ovarian cancer.Methods:Ovarian cancer tissue and normal tissue sequencing data were downloaded from the Cancer Genome Atlas(TCGA)and GTEx databases to analyze the correlation of DACT3 with overall survival(OS)and progression-free survival(PFS)in patients with ovarian cancer.Sixty cases of serous ovarian cancer and normal ovarian tissue were selected.Immunohistochemistry was performed to detect DACT3 expression in ovarian cancer tissues.Univariate and multifactorial COX analyses were performed to test the risk factor affecting the prognosis of ovarian cancer.Cell migration ability of SKOV3 was detected by cell scratch assay.Results:TCGA and GTEx database data displayed that OS and PFS were shorter in patients with high DACT3 expression(P<0.05);COX analyses suggested that DACT3 could be an independent prognostic predictor in ovarian cancer(P=0.00051).Analysis of 60 ovarian cancer patients showed that DACT3 expression was correlated with tumor differentiation and FIGO stage(P<0.05),and patients with high DACT3 expression had shorter OS.DACT3 was highly expressed in ovarian plasmacytoma,especially in high-grade types.Inhibition of DACT3 expression significantly inhibited cell migration.Conclusion:DACT3 is highly expressed in ovarian cancer,and is an independent risk factor affecting the prognosis of ovarian cancer.It may be used as a prognostic indicator and molecular therapeutic traget for ovarian cancer patients.

关 键 词：β-环连蛋白抑制基因3 卵巢癌 预后 

分 类 号：R737.31[医药卫生—肿瘤]