lncRNA UNC5B-AS1和miR-381在非小细胞肺癌组织中的表达及临床意义  

Expression and clinical significance of lncRNA UNC5B⁃AS1 and miR⁃381 in non⁃small cell lung cancer

作　　者：肖婧 度长海 田林 XIAO Jing;DU Changhai;TINA Lin(Department of Pathology,Renmin Hospital,Hubei Univesity of Medicine,Shiyan 442000,China)

机构地区：[1]十堰市人民医院(湖北医药学院附属人民医院)病理科,湖北十堰442000

出　　处：《临床肿瘤学杂志》2023年第9期806-811,共6页Chinese Clinical Oncology

摘　　要：目的探讨长链非编码RNA(lncRNA)非协调性分子5同源蛋白B-反义RNA1(UNC5B-AS1)和miR-381在非小细胞肺癌(NSCLC)组织中的表达及临床意义。方法采用实时荧光定量PCR(qPCR)检测NSCLC患者肿瘤组织(实验组)和肺良性肿瘤组织(对照组)lncRNA UNC5B-AS1和miR-381的表达差异;分析lncRNA UNC5B-AS1和miR-381的表达与NSCLC临床病理特征之间的关系;采用受试者工作特征(ROC)曲线评估lncRNA UNC5B-AS1和miR-381对NSCLC的诊断价值。采用多因素Cox回归模型分析影响NSCLC患者预后的独立因素。结果实验组lncRNA UNC5B-AS1的表达明显高于对照组(P<0001),而miR-381的表达明显低于对照组(P<0001)。lncRNA UNC5B-AS1和miR-381的表达呈明显负相关(r=-0352,P=0001)。lncRNA UNC5B-AS1、miR-381及两者联合诊断NSCLC的曲线下面积(AUC)分别为0860、0850和0924。LncRNA UNC5B-AS1与NSCLC患者的TNM分期、淋巴结转移及分化程度有关(P<005);miR-381与与NSCLC患者的肿瘤大小、淋巴结转移及分化程度有关(P<005)。lncRNA UNC5B-AS1高表达组患者的中位OS为269个月,明显低于低表达组的410个月(P<005);miR-381低表达组患者的中位OS为255个月,明显低于高表达组的420个月(P<005)。Cox多因素回归分析显示,肿瘤大小、TNM分期、淋巴结转移、lncRNA UNC5B-AS1表达及miR-381表达是影响NSCLC患者预后的独立因素(P<005)。结论在NSCLC组织中lncRNA UNC5B-AS1表达升高,而miR-381表达下降,两者对NSCLC有一定的诊断和预后预测价值。Objective To explore the expression and clinical significance of long noncoding RNA(lncRNA)uncoordinated-5-homolog B-antisense RNA 1(UNC5B-AS1)and miR-381 in non-small cell lung cancer(NSCLC).Methods The difference of lncRNA UNC5B-AS1 and miR-381 expression in tumor tissues(experimental group)and benign lung tumor tissues(control group)of NSCLC patients was detected by real-time fluorescent quantitative PCR(qPCR).The correlation between the expressions of lncRNA UNC5B-AS1,miR-381 and clinical characteristics was analyzed.The receiver operating characteristic curve(ROC)was used to evaluate the diagnostic value of lncRNA UNC5B-AS1 and miR-381 for NSCLC.Cox multivariate regression analysis was used to evaluate the risk factors affecting the prognosis of NSCLC patients.Results The lncRNA UNC5B-AS1 expression in the experimental group was significantly higher than that in the control group(P<0001),and the miR-381 expression was significantly lower than that in the control group(P<0001).The lncRNA UNC5B-AS1 was significantly negatively correlated with miR-381(r=-0352,P=0001).The area under the ROC curve of lncRNA UNC5B-AS1,miR-381 and their combined analysis for the diagnosis of NSCLC were 0860,0850 and 0924,respectively.LncRNA UNC5B-AS1 was significantly correlated with TNM stage,lymph node metastasis and degree of tissue differentiation(P<005),and miR-381 was significantly correlated with tumor size,lymph node metastasis and degree of tissue differentiation(P<005).The median overall survival(OS)of lncRNA UNC5B-AS1 high expression group was significantly lower than that of low expression group(269 months vs.410 months,P<005),and the median OS of miR-381 low expression group was significantly lower than that of high expression group(255 months vs.420 months,P<005).In addition,tumor size,TNM stage,lymph node metastasis,lncRNA UNC5B-AS1 expression and miR-381 expression were independent factors affecting the prognosis of NSCLC patients(P<005).Conclusion lncRNA UNC5B-AS1 was highly expressed,while miR-381 was low-expresse

关 键 词：非小细胞肺癌 非协调性分子5同源蛋白B-反义RNA1(UNC5B-AS1) miR-381 ROC曲线 

分 类 号：R734[医药卫生—肿瘤]