盐碱胁迫下燕麦叶片代谢组差异分析  

Differential analysis of oat leaf metabolism under salt and alkali stress

作　　者：李俊伟 刘景辉[1] 赵宝平[1] 米俊珍[1] 王俊英[3] 郭来春[2] 王春龙[2] 任长忠[2] LI Junwei;LIU Jinghui;ZHAO Baoping;MI Junzhen;WANG Junying;GUO Laichun;WANG Chunlong;REN Changzhong(National Agricultural Research Outstanding Talents and their Innovation Team/Inner Mongolia University Oat Engineering Research Center/Inner Mongolia Agricultural University Multigrain Industry Collaborative Innovation Center,Hohhot 010019,Inner Mongolia,China;Jilin Province Baicheng Academy of Agricultural Sciences,Baicheng 137000,Jilin,China;Institute of Biotechnology,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]全国农业科研杰出人才及其创新团队/内蒙古高校燕麦工程研究中心/内蒙古农业大学杂粮产业协同创新中心,内蒙古呼和浩特010019 [2]吉林省白城市农业科学院,吉林白城137000 [3]中国农业科学院生物技术研究所,北京100081

出　　处：《草业科学》2023年第10期2607-2618,共12页Pratacultural Science

基　　金：国家重点研发计划国际合作重点专项(2018YFE0107900);内蒙古重大专项(2021ZD0002);燕麦新品种选育、绿色栽培技术与营养功能产(2021CX0009);燕麦抗逆种质资源评价、新品种选育与利用(2021ZD0002);国家燕麦荞麦产业技术体系项目(CARS-07)。

摘　　要：以燕麦(Avena sativa)叶片为材料,结合广靶代谢组和靶向代谢组检测方法,分析了盐胁迫和碱胁迫条件下的主要差异代谢物及其代谢途径。结果发现:盐胁迫和碱胁迫条件下,燕麦叶片的主要差异代谢物均以脂类代谢物为主,有机酸是盐胁迫与碱胁迫之间的主要差异代谢物;富集分析表明,碱胁迫下的三羧酸循环(tricarboxylic acid cycle,TCA)代谢途径与盐胁迫条件下存在差别。通过检测TCA循环相关的有机酸,发现碱胁迫下燕麦叶片的柠檬酸、乌头酸、琥珀酸、延胡索酸、苹果酸、草酰乙酸含量高于盐胁迫,其中乌头酸含量明显高于其他有机酸。总之,TCA循环是盐胁迫和碱胁迫之间的主要差异代谢通路,燕麦叶片通过积累有机酸响应碱胁迫。本研究通过代谢组学方法首次证实了燕麦叶片响应盐、碱胁迫的主要差异代谢途径是TCA途径,推测乌头酸在燕麦叶片响应碱胁迫的有机酸调节过程中发挥主要作用,为解析燕麦响应盐、碱胁迫的差异机制奠定基础。To compare the metabolism of oat under salt and alkali stress,we analyzed the main differential metabolites and the metabolic pathways under salt and alkali stress conditions using wide target metabolome and targeted metabolome assays.The results showed that lipid metabolites were the main differential metabolites in oat leaves under both salt and alkali stress conditions,whereas the differential metabolites between salt and alkali stress were organic acids.Enrichment analysis showed that the tricarboxylic acid cycle(TCA)cycle is the main pathway that responds to alkali stress,but not to salt stress.Organic acids in the TCA cycle detected using a targeted metabolome assay showed that citric,aconitic,succinic,fumaroid,malic,and oxaloacetic acids were higher under alkali stress than under salt stress.Among these,the content of aconitic acid under alkali stress was significantly higher than that of other organic acids in the TCA cycle.In conclusion,organic acid metabolism is the major difference between salt and alkali stress,and the TCA cycle is the main pathway of organic acid metabolism.To our knowledge,this is the first study to use a metabolomic approach to confirm that the TCA cycle is the main metabolic pathway of oat leaves that differentiates the response to alkali and salt stress.Aconitic acid plays a major role in the process of organic acid regulation in oat leaves in response to alkali stress,and the results may lay the foundation for the analysis of the differential mechanisms of oat response to salt and alkali stress.

关 键 词：盐胁迫 碱胁迫 燕麦 有机酸 乌头酸 

分 类 号：S544.9[农业科学—作物学]