马铃薯转录因子StNAC043基因的克隆及其生理功能分析  

Cloning and physiological function analysis of potato transcription factor StNAC043 gene

作　　者：唐鑫华[1,2] 曲自成 李世伟 刘玉霖 石瑛 TANG Xinhua;QU Zicheng;LI Shiwei;LIU Yulin;SHI Ying*(Agricultural College,Northeast Agricultural University,Harbin 150030,China;National Research Center of Soybean Engineering and Technology,Harbin 150028,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]国家大豆工程技术研究中心,哈尔滨150028

出　　处：《中国农业大学学报》2023年第10期66-74,共9页Journal of China Agricultural University

基　　金：现代农业产业技术体系建设专项(CARS-09);寒地粮食作物种质创新与生理生态教育部重点实验室开放课题(CXSTOP202205)。

摘　　要：为探究马铃薯转录因子StNAC043基因的功能,克隆马铃薯转录因子StNAC043基因,利用农杆菌介导法将其转入马铃薯‘东农310’基因组,使其过量表达。转基因植株经无性系扩繁后,测定转基因株系的根和茎的生长指标、叶绿素相对含量(SPAD)以及荧光参数(Fv/Fm、ETR_(max)),并以转录组测序结合qRT-PCR验证分析其下游调控基因。结果表明:StNAC043基因过量表达可以显著促进转基因马铃薯根系和茎的生长、显著提高转基因植株叶片SPAD、Fv/Fm、ETR_(max);并且转录因子StNAC043可以调控捕光色素结合蛋白基因StCAB1和StCAB2的表达。综上,马铃薯转录因子StNAC043基因对马铃薯幼苗的生长和光合生理调控具有重要作用。To reveal the function of potato transcription factor StNAC043 gene,apotato transcription factor StNAC043 gene was cloned and transferred into potato‘Dongnong 310’genome by agrobacterium-mediated method to overexpress the gene.After clonal propagation of transgenic plants,the root and stem growth indexes,chlorophyll relative content(SPAD)and fluorescence parameters(Fv/Fmand ETR_(max))of transgenic lines were measured,and the downstream regulatory genes were verified by transcriptome sequencing combined with qRT-PCR.The results showed that the overexpression of StNAC043 gene significantly promoted the growth of roots and stems of transgenic potato,significantly increased the SPAD,Fv/Fm and ETR_(max)of transgenic plants,and the transcription factor StNAC043 regulated the expression of light-harvesting pigment binding protein genes StCAB1 and StCAB2.In conclusion,the potato transcription factor StNAC043 gene plays an important role in potato seedling growth and photosynthetic physiological regulation.

关 键 词：马铃薯 转录因子 StNAC043 根系 荧光参数 

分 类 号：S532[农业科学—作物学]