肝细胞癌患者中CAC1、CCNB2、Cyclin Y及Cyclin E1的表达及意义  

Expression and clinical significance of CAC1, CCNB2, Cyclin Y and Cyclin E1 in patients with hepatocellular carcinoma

作　　者：方丽 牛广旭[1] 吴士茜[1] 王静[1] FANG Li;NIU Guang-xu;WU Shi-qian;WANG Jing(Department of Pathology,Handan Central Hospital,Handan 056000,Hebei,China)

机构地区：[1]邯郸市中心医院病理科,河北邯郸056000

出　　处：《广东医学》2023年第9期1114-1120,共7页Guangdong Medical Journal

基　　金：河北省医学科学研究重点课题计划项目(201801860)。

摘　　要：目的 研究肝细胞癌(HCC)组织中细胞周期素依赖激酶2相关性Culin结构域1(CAC1)、细胞周期蛋白B2(CCNB2)、细胞周期蛋白Y(Cyclin Y)及细胞周期蛋白E1(Cyclin E1)的表达,探讨四者的临床意义。方法 应用R语言分析癌症基因组图谱数据库中HCC癌和癌旁组织中CAC1、CCNB2、Cyclin Y及Cyclin E1 mRNA的表达。免疫组化检测诊治的84例HCC癌组织及癌旁组织中CAC1、CCNB2、Cyclin Y及Cyclin E1蛋白表达。Pearson相关分析癌组织中各指标表达的相关性。统计学分析HCC癌组织中CAC1、CCNB2、Cyclin Y及Cyclin E1表达与临床病理参数的关系。Kaplan-Meier生存分析(Log-rank检验)分析CAC1、CCNB2、Cyclin Y及Cyclin E1表达与HCC患者生存预后的关系。结果 TCGA数据库HCC癌组织中CAC1、CCNB2、Cyclin Y及Cyclin E1 mRNA的表达显著高于癌旁组织,差异有统计学意义(P<0.05)。Pearson相关分析结果CAC1、CCNB2、Cyclin Y与Cyclin E1 mRNA表达均呈正相关(P<0.05)。癌组织中CAC1、CCNB2、Cyclin Y及Cyclin E1蛋白阳性表达率明显高于癌旁组织(P<0.05)。HCC患者不同肿瘤TNM分期及肿瘤数目的癌组织中CAC1、CCNB2、Cyclin Y及Cyclin E1蛋白表达差异有统计学意义(P<0.05)。84例患者随访2~60个月,随访期间死亡39例,失访2例,5年总体生存率52.44%(43/82)。CAC1、CCNB2、Cyclin Y及Cyclin E1阳性表达组的5年总体生存率分别低于阴性表达组患者(P<0.05)。结论 HCC中CAC1、CCNB2、Cyclin Y及Cyclin E1表达升高,四者表达与肿瘤TNM分期及肿瘤数目有关,有助于判断HCC患者的生存预后。Objective To investigate the expression of cell cycle kinase 2-associated Cullin 1(CAC1),Cyclin B2(CCNB2),Cyclin Y,and Cyclin E1 in hepatocellular carcinoma(HCC)tissues and explore their clinical significance.Methods The expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1 mRNA in HCC and adjacent normal tissues was analyzed using the Cancer Genome Atlas(TCGA)database.Immunohistochemistry was used to detect the protein expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1 in 84 cases of HCC tissues and adjacent tissues.Pearson correlation analysis was performed to assess the correlation of these markers′expression in cancer tissues.Statistical analysis was conducted to investigate the relationship between the expression of CAC1,CCNB2,Cyclin Y,Cyclin E1,and clinical pathological parameters.Kaplan-Meier survival analysis(Log-rank test)was used to assess the relationship between the expression of these markers and the prognosis of HCC patients.Results The expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1 mRNA in HCC tissues from the TCGA database was significantly higher than in adjacent normal tissues,with statistical significance(P<0.05).Pearson correlation analysis showed a positive correlation between the mRNA expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1(P<0.05).The positive expression rate of CAC1,CCNB2,Cyclin Y,and Cyclin E1 proteins in HCC tissues was significantly higher than in adjacent tissues(P<0.05).The protein expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1 in HCC tissues varied significantly among patients with different TNM stages and tumor numbers(P<0.05).Among 84 patients followed for 2-60 months,there were 39 deaths,2 lost to follow-up,and a 5-year overall survival rate of 52.44%(43/82).Patients with positive expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1 had lower 5-year overall survival rates compared to those with negative expression(P<0.05).Conclusion The expression of CAC1,CCNB2,Cyclin Y,and Cyclin E1 is upregulated in HCC,and their expression is associated with TNM staging and the number of tumors.T

关 键 词：肝细胞癌 细胞周期素依赖激酶2相关性Culin结构域1 细胞周期蛋白B2 细胞周期蛋白Y 细胞周期蛋白E1 临床病例特征 预后 

分 类 号：R735.7[医药卫生—肿瘤] R44[医药卫生—临床医学]