鼻咽癌组织中BMI1和ERK3的表达及其临床意义  

Expression and clinical significance of BMI1/ERK3 in nasopharyngeal carcinoma tissues

作　　者：李民英[1] 闫新玉 王婷[1] 曾玉梅[3] 张晶晶[1] 郑斯明[1] 周嘉雄 李娅妮 查雅文 文磊 LI Min-ying;YAN Xin-yu;WANG Ting;ZENG Yu-mei;ZHANG Jing-jing;ZHENG Si-ming;ZHOU Jia-xiong;LI Ya-ni;ZHA Ya-wen;WEN Lei(Department of Radiotherapy,Cancer Branch,Zhongshan People′s Hospital,Zhongshan 528400,Guangdong,China;不详)

机构地区：[1]中山市人民医院肿瘤分院放疗科,广东中山528400 [2]新乡医学院研究生院,河南新乡453003 [3]中山市人民医院肿瘤分院病理科,广东中山528400 [4]中山大学肿瘤医院内科,广东广州510060 [5]新乡医学院 [6]中山市人民医院

出　　处：《广东医学》2023年第8期1025-1031,共7页Guangdong Medical Journal

基　　金：中山市社会公益与基础研究项目(医疗卫生)(2020B1079)。

摘　　要：目的探讨鼻咽癌(NPC)组织中BMI1和ERK3的表达、两者的相关性及其在NPC侵袭转移中的作用。方法检索并下载GEO数据库中NPC测序基因集GSE103611和GSE34573,分析BMI1和ERK3基因表达数据。采用免疫组化MaxVision法检测51例NPC患者组织和18例慢性鼻咽炎组织中BMI1和ERK3的表达情况,结合随访资料分析以上指标的临床意义。结果BMI1在NPC组织和慢性鼻咽炎组织中的阳性率分别是56.9%和22.2%,ERK3在NPC组织和慢性鼻咽炎组织中的阳性率分别是52.9%和11.1%。BMI1和ERK3在NPC组织中的阳性率均高于慢性鼻咽炎组织(P<0.05),尤其在NPC的N2~3组中两者呈显著高表达。BMI1与ERK3的表达呈正相关(r_(s)=0.345,P<0.05)。同时在NPC组织中BMI1和ERK3的表达与T分期、N分期以及总分期有关(P<0.05),与患者性别、初诊血清EBV DNA拷贝数无关(P>0.05)。结论BMI1/ERK3通路可在NPC的侵袭转移中发挥一定的作用。Objective To investigate the expression and correlation between BMI1 and ERK3 in nasopharyngeal carcinoma(NPC)tissues,and their roles in the invasive metastasis of NPC.Methods The NPC sequence gene sets GSE103611 and GSE34573 in the GEO database were retrieved and downloaded to analyze BMI1 and ERK3 gene expression.And the expression of BMI1 and ERK3 in the tissues of 51 patients with NPC and 18 patients with chronic rhinopharyngitis were assessed by the immunohistochemical in MaxVision method,and the clinical value of the above indexes was analyzed together with the follow-up data.Results The positive rates of BMI1 in NPC tissues and chronic nasopharyngitis tissues were 56.9%and 22.2%,respectively;while the positive rates of ERK3 in NPC tissues and chronic nasopharyngitis tissues were 52.9%and 11.1%,respectively.Both BMI1 and ERK3 were significantly more positive in NPC tissues than in chronic nasopharyngitis tissues(P<0.05),and both were significantly higher expressed in the NPC N2-3 group particularly.The expression of BMI1 and ERK3 had a significant positive correlation(r_(s)=0.458,P<0.05).Meanwhile,the expression of BMI1 and ERK3 in NPC tissues was correlated with T-stage,N-stage,and total stage(P<0.05),but not with patient gender,or initial serum EBV DNA copy number(P>0.05).Conclusion The BMI1/ERK3 pathway may play roles in the invasive metastasis of NPC.

关 键 词：鼻咽癌 BMI1 ERK3 免疫组织化学 转移/侵袭 

分 类 号：R739.63[医药卫生—肿瘤] R392.3[医药卫生—临床医学]