酢浆草对去势抵抗性前列腺癌模型小鼠肿瘤抑制作用及其机制研究  

Oxalis inhibits prostate tumor in the mouse model of castration-resistant prostate cancer:Effect and mechanism

作　　者：娄强 杨丽 肖友平 商学军 LOU Qiang;YANG Li;XIAO You-ping;SHANG Xue-jun(Department of Urology,The Second Affiliated Hospital of Guizhou University of Chinese Medicine,Guiyang,Guizhou 550001,China;Department of Urology,Jinling Hospital Affiliated to Nanjing University School of Medicine/General Hospital of Eastern Theater Command,Nanjing,Jiangsu 210002,China)

机构地区：[1]贵州中医药大学第二附属医院泌尿外科,贵州贵阳550001 [2]南京大学医学院附属金陵医院/东部战区总医院泌尿外科,江苏南京210002

出　　处：《中华男科学杂志》2023年第2期106-112,共7页National Journal of Andrology

基　　金：贵州省科学技术厅基础研究计划项目(201842924832410496)。

摘　　要：目的:探讨酢浆草调控NF-κB对去势抵抗性前列腺癌(CRPC)模型小鼠肿瘤抑制作用及机制。方法:将40只6~8周龄C57/BL雄性小鼠建立CRPC裸鼠模型后随机分为生理盐水对照组[0 mg/(kg·d)]、酢浆草低剂量组[5 mg/(kg·d)]、酢浆草中剂量组[10 mg/(kg·d)]和酢浆草高剂量组[15 mg/(kg·d)],早晚各灌胃1次,连续灌胃28 d。测量各组CRPC裸鼠的前列腺肿瘤体积和平均重量,计算各组CRPC裸鼠抑瘤率。采用HE染色法分析前列腺肿瘤组织形态学变化,采用免疫荧光检测CRPC模型前列腺肿瘤组织NF-κB通路及下游蛋白表达。结果:与对照组相比,酢浆草低、中、高剂量组肿瘤细胞密度逐渐降低,细胞退变逐渐明显,前列腺肿瘤坏死细胞逐渐增多。与对照组相比,酢浆草低、中、高剂量组前列腺肿瘤体积及平均重量依次递减(P<0.05)。与对照组相比,酢浆草低、中、高剂量组NF-κB p65、Ki67阳性表达逐渐下调(P<0.05),而Bax阳性表达逐渐上调(P<0.05)。结论:酢浆草可抑制CRPC模型小鼠前列腺肿瘤体积和平均重量的生长,其机制可能和酢浆草通过下调NF-κB通路,下调p65、Ki67表达,上调Bax表达,促进CRPC模型肿瘤细胞退变及坏死有关。Objective:To investigate the inhibitory effect of oxalis on prostate tumor in the mouse model of castration-resistant prostate cancer(CRPC)and its action mechanismM.ethods:We established a CRPC model in 40 male C57/BL mice aged 6-8 weeks,divided them randomly into four groups of an equal number,and treated them intragastrically with normal saline(control),low-dose oxalis(5 mg/kg/d),medium-dose oxalis(10 mg/kg/d),and high-dose oxalis(15 mg/kg/d),respectively.After 28 days of treatment,we measured the tumor volume and body weight of the mice in different groups,calculated the tumor-inhibition rate,examined the histomorphological changes of the prostate tumors by HE staining,and detected the expressions of the nuclear factor-kB(NF-kB)signaling pathway and its downstream proteins in the tumor tissue by immunofluorescence assay.Results:In comparison with the controls,the mice in the low-,medium-and high-dose oxalis groups showed a gradual decrease in tumor cell concentration and cell degeneration,and a gradually increased number of necrotic tumor cells.The volume and mean weight of prostate tumors were significantly reduced(P<0.05),the expressions of NF-kB p65 and Ki67 proteins remarkably down-regulated(P<0.05),and that of the Bax protein markedly up-regulated(P<0.05)in the oxalis groups compared with the controls.Conclusion:Oxalis can inhibit the growth of prostate tumor in CRPC mice possibly by down-regulating the NF-kB signaling pathway and the expressions of p65 and Ki67 and up-regulating the expression of Bax,and thereby promoting the degeneration and necrosis of tumor cells.

关 键 词：酢浆草 去势抵抗性前列腺癌 NF-ΚB信号通路 P65 KI67 Bax 

分 类 号：R737.25[医药卫生—肿瘤]