一株支气管炎鲍特杆菌国家标准菌种的再鉴定  

Re-Identification of a National Standard Strain Seed of Bordetella bronchiseptica

作　　者：石继春[1] 龙新星 陈驰[1] 梁丽[1] 郑锐 孙文媛 杜慧竟[1] 徐潇[1] 叶强[1] SHI Ji-chun;LONG Xin-xing;CHEN Chi;LIANG Li;ZHENG Rui;SUN Wen-yuan;DU Hui-jing;XU Xiao;YE Qiang(Nat'l Inst.for Food&Drug Ctrl.,Nat'l Ctr.for Med.Culture Collections,NHC Key Lab.of Res.on Qual.&Standard'n of Biotech Products,NMPA Key Lab.for Qual.Res.&Evaluat'n of Bio-Products,Beijing 102629)

机构地区：[1]中国食品药品检定研究院、中国医学细菌保藏管理中心、国家卫生健康委员会生物技术产品检定方法及其标准化重点实验室、国家药品监督管理局生物制品质量研究与评价重点实验室,北京102629

出　　处：《微生物学杂志》2023年第3期69-79,共11页Journal of Microbiology

基　　金：国家病原微生物资源库项目(国家病原微生物资源库-NPRC-32)。

摘　　要：通过生化试验、质谱鉴定、16S rRNA基因序列分析、全基因组序列测定及生物信息学分析等方法对国家标准菌种CMCC(B)40001进行再鉴定。对菌株的毒力、耐药和MLST进行分析,并对鲍特杆菌(Bordetella spp.)的群体进化进行分析。结合不同方法,CMCC(B)40001再鉴定为支气管炎鲍特杆菌(Bordetella bronchiseptica),且其作为标准菌种能够发挥国家标准描述的质控作用。同时获取该菌株的新ST型,在进化关系上,其与百日咳鲍特杆菌(Bordetella pertussis)和副百日咳鲍特杆菌(Bordetella parapertussis)遗传距离近。本研究再鉴定了国家标准菌种CMCC(B)40001为支气管炎鲍特杆菌,并分析了其遗传特征,为国家标准菌种的应用提供资源描述支持。A national standard strain CMCC(B)40001 was re-identified through biochemical tests,mass spectrometry identification,16S rRNA gene sequence analysis,whole genome sequence determination and bioinformatics analysis.The virulence of the strain,drug resistance and MLST of the strain,the evolutionary relationship of Bordetella spp.The results showed that combining different methods,CMCC(B)40001 was re-identified as Bordetella bronchiseptica.And as a standard strain,it could play the role of quality control described in national standard.At the same time,a new ST type of this strain was obtained.In terms of evolutionary relationship,it is genetically close to Bordetella pertussis and Bordetella parapertussis.To conclude,in this study,the national standard strain CMCC(B)40001 was reidentified as Bordetella bronchiseptica,and its genetic characteristics was analyzed to provide resource description support for the application of this national standard strain.

关 键 词：细菌鉴定 质谱鉴定 基因组测序 群体进化分析 质量控制 

分 类 号：Q93-331[生物学—微生物学]