NH_(3)和H_(2)S降解功能菌的分离鉴定及降解特性研究  

Isolation,Identification and Degradation Characteristics of Functional Bacteria for NH_(3)and H_(2)S Degradation

作　　者：江海溶 崔若琪 王玥 白淼 张明露[1] 任连海[1] JIANG Hai-rong;CUI Ruo-qi;WANG Yue;BAI Miao;ZHANG Ming-lu;REN Lian-hai(State Environmental Protection Key Laboratory of Food Chain Pollution Control,Beijing Technology and Business University,Beijing 100048)

机构地区：[1]北京工商大学生态环境学院国家环境保护食品链污染防治重点实验室,北京100048

出　　处：《生物技术通报》2023年第9期246-254,共9页Biotechnology Bulletin

基　　金：国家重点研发计划(2019YFC1906004)。

摘　　要：为了减少厨余垃圾堆放时恶臭气体的排放,本研究利用富集驯化、定性初筛和定量复筛法从厨余垃圾中分离筛选到2株对NH3和H2S均具有高效降解作用的菌株CN5和CS2。通过16S rDNA序列分析,分别鉴定为肠球菌属(Enterococcuss sp.)和假单胞菌属(Pseudomonas sp.)。通过单因素实验,确定两株菌的最佳除臭条件为:培养温度为35℃、pH为7、碳氮比25∶1、碳源为蔗糖、氮源为氯化铵,对NH_(3)和H_(2)S的降解率均能达到85%左右。菌株CN5和CS2在2∶3的混合状态下生长状况最好,p H值稳定,OD_(600)最大值为1.342。通过氨氮含量和硫酸盐含量测定实验发现,最终氨氮含量为50.5 mg/L,菌剂对氨氮降解率为85.3%;硫酸盐含量为302.7 mg/L,菌剂对硫酸盐生成率为89.2%。结果表明,从厨余垃圾中筛选得到的2株菌对NH_(3)和H_(2)S具有良好的降解能力,且两菌株相互之间具有协同作用。In order to reduce the emission of malodorous gas during the stacking of kitchen waste,two strains CN5 and CS2 with high degradation of NH3 and H2S were isolated and screened from kitchen waste by enrichment and domestication,qualitative primary screening and quantitative secondary screening methods.They were identified as Enterococcus sp.and Pseudomonas sp.by 16S rDNA sequence analysis.The optimal deodorizing conditions of microbial deodorant were determined by single factor experiment:culture temperature was 35℃,pH was 7,carbon nitrogen ratio was 25∶1,carbon source was sucrose,nitrogen source was ammonium chloride,and the degradation rate of NH_(3)and H_(2)S could be maintained at about 85%.Strains CN5 and CS2 grew best in the mixed state of 2∶3,and the maximum value of OD_(600)was 1.342.The determination of ammonia nitrogen content and sulfate content,it was found that the final ammonia nitrogen content was 50.5 mg/L,and the degradation rate of bacteria to ammonia nitrogen was 85.3%.The sulfate content was 302.7 mg/L,and the sulfate production rate of bacterial agent was 89.2%.The results showed that the two strains screened from the kitchen waste had good ability to degrading NH_(3)and H_(2)S,and the two strains had synergistic effect with each other.

关 键 词：NH3降解菌 H2S降解菌 筛选 鉴定 优化 

分 类 号：X172[环境科学与工程—环境科学] X799.3