融合免疫荧光技术的共聚焦显微镜实验教学实践  

Construction and Practice of Experimental Teaching on Confocal Microscopy with Immunofluorescence Technique

作　　者：刘如明[1] 边鑫 龙加福[1] 周颖[1] 缴莉 LIU Ruming;BIAN Xin;LONG Jiafu;ZHOU Ying;JIAO Li(College of Life Sciences,Nankai University,Tianjin 300071,China)

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《实验室研究与探索》2023年第7期229-232,共4页Research and Exploration In Laboratory

基　　金：教育部高等教育司基础学科拔尖学生培养计划2.0研究项目(20212017)。

摘　　要：为了建立完整的科研实验技能学习体系,构建了高效可行的实验教学课程,并对近年开展的激光扫描共聚焦显微镜技术实验教学课程内容和方法进行了优化和实践。课程将科学理论与实验技能有机结合,把间接免疫荧光技术融合到共聚焦显微镜实验教学中,克服大型成像仪器教学中时间和空间限制。该教学课程帮助学生掌握共聚焦显微镜技术原理、应用和实验方法,提高学生们的实验动手能力和科研素养。In order to establish a complete learning system of scientific research skills and an efficient and feasible course,the teaching content and method of laser scanning confocal microscopy are optimized and practiced.Technical training is combined with theoretical teaching and immunofluorescence technique is integrated into the course,which could help overcome the limitations of time and space on large-instrument teaching.The optimized course aims to guide students to master the principle,application and technique of confocal microscopy and improve their practical ability and scientific research capacity.

关 键 词：激光扫描共聚焦显微镜 免疫荧光技术 实验教学 

分 类 号：G642.0[文化科学—高等教育学]