大黄鱼Cspg4基因的表达调控  

Expression Regulation of Cspg4 in Large Yellow Croaker

作　　者：罗阿蓉 李完波 王志勇[1] LUO Arong;LI Wanbo;WANG Zhiyong(Fisheries College&Key Laboratory of Healthy Mariculture for East China Sea of Ministry Agriculture and Rural Affairs,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学水产学院、农业农村部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2023年第4期298-308,共11页Journal of Jimei University：Natural Science

基　　金：国家自然科学基金项目(31872562);海水鱼类产业技术体系项目(CARS-47-G04)。

摘　　要：从大黄鱼脾脏eQTL数据库中,筛选出调控免疫相关靶基因Cspg4的顺式作用区eQTL,通过Sanger测序验证了该eQTL区域中最显著的SNP位点的基因型。在此基础上,通过构建候选启动子区不同长度缺失片段的重组质粒测定活性,确定了Cspg4基因启动子核心区为-1202~-762和-190~+20,而携带Chr17_13952653突变位点的两种单倍型调控序列的活性均显著低于对照组,提示该调控序列可能为沉默子。In this study,the Cspg4 gene was cloned based on previous eQTL analysis in Larimichthys crocea,which was regulated by cis-eQTLs.The results of Sanger sequencing verified the genotypes of the most significant SNP Chr17_13952653 regulating Cspg4 gene.Subsequently,the core promoter regions of Cspg4 were determined to be from-1202 to-762 and from-190 to+20 through recombinant plasmids with different truncated fragments in the candidate promoter region.Further study found that the activity of the two haplotypes containing Chr17_13952653 site was significantly lower than that of the control group,suggesting that the sequence may be a silencer,and weaken the promoter activity in transcription.Our results provided useful information for elucidating the molecular mechanisms of cis-regulation of Cspg4 gene in L.crocea.

关 键 词：大黄鱼 Cspg4 表达调控 沉默子 

分 类 号：S963[农业科学—水产养殖]