油用向日葵种子发育过程中油酸形成的转录组学分析  

Transcriptome Analysis of Oleic Acid Formation during Seed Development of Oil Sunflower

作　　者：李洋[1] 杨翔宇 任孝慈 李晓伟[1] 郭荣秋 何中国[1] 王佰众[1] 张连喜[1] LI Yang;YANG Xiangyu;REN Xiaoci;LI Xiaowei;GUO Rongqiu;HE Zhongguo;WANG Baizhong;ZHANG Lianxi(Institute of Peanut Research,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China)

机构地区：[1]吉林省农业科学院花生研究所,吉林公主岭136100

出　　处：《东北农业科学》2023年第4期22-27,50,共7页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科技创新工程项目(CXGC2021ZY107);国家特色油料产业技术体系项目(CARS-14-2-12)。

摘　　要：为探索油用向日葵油酸品质形成的调控规律,以高油酸材料NY7和低油酸材料N96为研究对象,分别对开花后7、14、21、25、28、31、35、42 d种子取样,测定不同生育期种子百粒重及油酸含量变化,并采用RNA-Seq技术对开花后21、25、31 d的种子进行转录组测序。结果表明,高、低油酸向日葵种子在开花后21 d均基本完成了干物质的积累;低油酸种子开花后14 d油酸开始降解,油酸含量逐渐降低;转录组测序分别获得8955、7664、9287个差异表达基因;对差异基因进行KEGG富集分析,分别富集在33、16、32条代谢通路,其中与油酸形成相关的通路为脂肪酸降解代谢通路,在这条通路中筛选到了8个与油酸形成相关的候选基因。To explore the regulation of oleic acid quality formation of oil sunflower,the high oleic acid material NY7 and the low oleic acid material N96 were used as the research materials.The seeds were sampled at 7,14,21,25,28,31,35 and 42 days after flowering,respectively.The 100-seed weight and oleic acid content of seeds at different growth stages were measured.Additionally,RNA-Seq technology was employed to conduct the transcriptome sequence of the seeds at 21,25,and 31 days after flowering.The results showed that both high and low oleic acid sunflower seeds basically completed dry matter accumulation 21 days after flowering.In low oleic acid seeds,the degradation of oleic acid commenced after 14 days of flowering,leading to a gradual decrease in its content.Transcriptome sequencing obtained 8955,7664,and 9287 differentially expressed genes,respectively.KEGG enrichment analysis was conducted on differential genes,which were enriched in 33,16 and 32 metabolic pathways,among which the pathway related to oleic acid formation was fatty acid degradation metabolic pathway.Within this pathway,8 candidate genes potentially involved in oleic acid formation were identified.

关 键 词：向日葵 RNA-SEQ 油酸 种子 

分 类 号：S565.5[农业科学—作物学]