青藤碱通过调控STAT3及NF-κB信号通路对多发性骨髓瘤U266细胞增殖、凋亡的影响  

The effect of sinomenine on proliferation and apoptosis of multiple myeloma U266 cells through regulation of STAT3 and NF-κB signaling pathways

作　　者：王莹莹 周世颖 陈洁[2] 乔璎 赵逸 吴佳玙 吴英理 翁巍[2] Wang Yingying;Zhou Shiying;Chen Jie;Qiao Ying;Zhao Yi;Wu Jiayu;Wu Yingli;Weng Wei(Dept of Pathophysiology,Shanghai Jiaotong University School of Medicine,Key Laboratory of Cell Differentiation and Apoptosis,Ministry of Education,Shanghai 200025;Dept of Blood Transfusion,Tongren Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200336)

机构地区：[1]上海交通大学医学院病理生理学系细胞分化与凋亡教育部重点实验室,上海200025 [2]上海交通大学医学院附属同仁医院输血科,上海200336

出　　处：《安徽医科大学学报》2023年第10期1634-1638,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82170145)。

摘　　要：目的探讨青藤碱对骨髓瘤U266细胞增殖、凋亡的影响及其可能机制。方法将培养好的U266细胞用不同浓度青藤碱(0、0.5、1、2 mmol/L)处理,空白对照组加入0.5%浓度的DMSO。用CCK-8检测U266细胞的增殖;流式细胞术检测细胞的凋亡;Western blot法检测各组细胞的增殖相关蛋白、凋亡相关蛋白、信号转导与转录激活因子(STAT3)及基因结合核因子(NF-κB)信号通路相关蛋白的表达水平。结果与空白对照组比较,青藤碱处理组U266细胞凋亡率增加,增殖能力降低,抗凋亡相关蛋白Bcl-2及Mcl-1表达水平下降、cleaved caspase-3、cleaved PARP表达水平上调,STAT3、NF-κB信号通路活性降低,表现为p-STAT3、p-IκBα的表达水平下降。结论青藤碱通过下调STAT3及NF-κB信号通路活性,促进骨髓瘤U266细胞凋亡。Objective To investigate the effect of sinomenine on proliferation and migration of multiple myeloma(MM)cells by regulating STAT3 and NF-κB signaling pathway.Methods The cultured U266 cells were treated with different concentrations of sinomenine(0,0.5,1,2 mmol/L).The control group was added DMSO with 0.5%concentration.CCK-8 assay was used to detect the proliferation of U266 cells.Flow cytometry was used to detect the apoptosis of U266 cells.Western blot assay was used to detect the expression levels of apoptosis-related proteins,STAT3 and NF-κB signaling pathway proteins in the each group.Results Compared with CON group,the apoptosis of U266 cells increased after Sinomenine treatment,the proliferation was inhibited;B lymphoma-2(Bcl-2)myeloid and cell leukemia-1(Mcl-1)expression level decreased;activated Caspase-3(cleaved Caspase-3)and PARP(Cleaved Caspase-3)expression levels increased;the activity of STAT3 and NF-κB signaling pathway decreased.Conclusion Sinomenine can down-regulate the activity of STAT3 and NF-κB signaling pathway,promote the apoptosis of U266 cells and inhibit the proliferation of U266 cells.

关 键 词：青藤碱 骨髓瘤 U266细胞 STAT3信号通路 NF-ΚB信号通路 

分 类 号：R363.1[医药卫生—病理学]