HC2作为急性缺血性脑卒中诊断的潜在生物标志物  

Heparin Cofactor Ⅱ Is a Potential Biomarker for the Diagnosis of Acute Ischemic Stroke

作　　者：鲁莹 朱彧[2] 刘丽 李玲 张连文 Lu Ying;Zhu Yu;Liu Li;Li Ling;Zhang Lianwen(College of Pharmacy,Tianjin Key Laboratory of Molecular Drug Research,Nankai University,Tianjin 300353,China;Department of Clinical Laboratory,Tianjin Huanhu Hospital,Tianjin 300350,China)

机构地区：[1]南开大学药学院,天津市分子药物研究重点实验室,天津300353 [2]天津市环湖医院检验科,天津300350

出　　处：《南开大学学报（自然科学版）》2023年第3期34-38,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(31470795);中央高校基本科研业务费专项基金(南开大学63191148)。

摘　　要：为了探究急性缺血性脑卒中(Acute ischemic stroke,AIS)患者和对照组静脉血血清中的O-GlcNAc糖蛋白差异,筛选AIS诊断的潜在生物标志物.首先对AIS和对照组血清中的O-GlcNAc糖基化修饰进行检测,发现AIS的O-GlcNAc修饰程度明显高于对照组(P<0.05),通过CpOGAD298N富集AIS和对照组血清中的OGlcNAc蛋白并进行质谱鉴定,得到差异蛋白肝素辅助因子Ⅱ(Heparin Cofactor Ⅱ,HC2),Western blot检测结果表明,富集蛋白中HC2丰度在AIS组显著高于对照组(P<0.01),ROC曲线分析显示,HC2对急性缺血性脑卒中具有潜在的诊断价值(AUC=0.882),并且高于常用的血脂指示标志.To explore the difference between O-GlcNAcylated proteins in the serum of acute ischemic stroke(AIS)patients and the control group,and to screen the potential biomarkers for AIS diagnosis,the OGlcNAcylation in the serum of AIS and the control group was detected.The O-GlcNAcylation level in AIS was significantly higher than that in the control group(P<0.05).CpOGA(D298N)was used to enrich OGlcNAcylated proteins in the serum,and the differential protein HC2 was confirmed by mass spectra analyses and western blot.It was found that the amount of enriched HC2 in AIS was significantly higher than that in the control group(P<0.01).The enriched HC2 has a potential diagnostic value for acute ischemic stroke(AUC=0.882),which is better than common blood lipid indicators.

关 键 词：急性缺血性脑卒中 O-GlcNAc糖蛋白 HC2 潜在生物标志物 

分 类 号：R741[医药卫生—神经病学与精神病学]