上皮细胞转化序列2在浸润性乳腺癌中的表达及其预后意义  

Expression and prognostic significance of epithelial cell transforming sequence 2 in invasive breastcancer

作　　者：林智平 刘金发 肖晓煜 孟加榕[1] Lin Zhiping;Liu Jinfa;Xiao Xiaoyu;Meng Jiarong(Department of Pathology,the 909th Hospital of Joint Logistics Support Force,Dongnan Hospital of Xiamen University,School of Medicine,Xiamen University,Zhangzhou 363000,China;Department of Cardiovascular,the 909th Hospital of Joint Logistics Support Force,Dongnan Hospital of Xiamen University,School of Medicine,Xiamen University,Zhangzhou 363000,China)

机构地区：[1]联勤保障部队第九〇九医院厦门大学附属东南医院病理科,漳州363000 [2]联勤保障部队第九〇九医院厦门大学附属东南医院心血管内科,漳州363000

出　　处：《中国医师进修杂志》2023年第9期780-785,共6页Chinese Journal of Postgraduates of Medicine

基　　金：福建省医学创新课题项目(2018-CX-40);联勤保障部队第九O九医院青年苗圃基金(18Y017)。

摘　　要：目的:探讨上皮细胞转化序列2(ECT2)在浸润性乳腺癌中的表达及其预后意义。方法:回顾性分析2018年1月至2021年6月联勤保障部队第九〇九医院收治的165例浸润性乳腺癌患者手术治疗的临床资料,并收集患者的肿瘤组织和对应癌旁正常组织。对乳腺癌肿瘤组织和癌旁正常组织进行ECT2免疫组织化学染色。分析不同组织中ECT2蛋白表达情况与病理和临床资料关系。使用多因素Logistic回归分析影响ECT2蛋白高表达的因素。利用Kmplot在线生存分析公共数据库,使用Kaplan-Meier法比较ECT2高表达与ECT2低表达乳腺癌患者的生存时间差异。结果:乳腺癌组织中的ECT2表达比例高于癌旁正常组织[57.58%(95/165)比12.73%(21/165)],差异有统计学意义( χ^(2)= 76.19, P<0.01)。ECT2高表达组患者的病理组织学分级更差[G 1级30.53%(29/95)比57.14%(40/70),G 2级40.00%(38/95)比28.57%(20/70),G 3级29.47%(28/95)比14.20%(10/40)]、存在淋巴结转移更高[71.58%(68/95)比7.14%(5/70)]、TNM分期更差[Ⅰ期15.79%(15/95)比34.29%(24/70),Ⅱ期27.37%(26/95)比27.14%(19/70),Ⅲ期41.05%(39/95)比28.57%(20/70),Ⅳ期15.79%(15/95)比10.00%(7/70)]、绝经比例更高[60.00%(57/95)比44.29%(31/70)]和Ki-67增殖指数更高[74.74%(71/95)比58.57%(41/70)]( P均<0.05)。淋巴结转移( OR = 2.764, P = 0.038)、病理组织学分级为G 3级( OR = 1.942, P = 0.010)、TNM分期为Ⅳ期( OR = 2.586, P<0.001)、Ki-67增殖指数≥14%( OR = 1.376, P = 0.006)是乳腺癌患者肿瘤组织ECT2蛋白高表达的影响因素。ECT2高表达乳腺癌患者生存期更差,差异有统计学意义( P<0.05)。 结论:浸润性乳腺癌肿瘤组织中ECT2蛋白表达增加。ECT2高表达的乳腺癌患者预后更差,ECT2可能是乳腺癌治疗的潜在分子靶点,值得进一步研究。Objective To investigate the expression and prognostic significance of epithelial cell transforming sequence 2(ECT2)in invasive breast cancer.Methods The clinical data of 165 cases of invasive breast cancer treated in the 909th Hospital of Joint Logistics Support Force from January 2018 to June 2021 were retrospectively analyzed,the tumor tissues and corresponding normal tissues adjacent to the cancer were collected.The tumor tissues of breast cancer and adjacent normal tissues were stained with ECT2 immunohistochemistry.The relationship between the expression of ECT2 protein in different tissues and pathological and clinical data was analyzed.Multivariate Logistic regression was used to analyze the factors affecting the high expression of ECT2 protein.Kaplan-Meier method was used to compare the total survival time of breast cancer patients with high expression of ECT2 and low expression of ECT2 by Kmplot online survival analysis of public databases.Results The expression of ECT2 in breast cancer tissues was significantly higher than that in adjacent normal tissues:57.58%(95/165)vs.12.73%(21/165)(=76.19,P<0.01).The patients with high expression of ECT2 had worse histopathological grade:grade G,30.53%(29/95)vs.57.14%(40/70),grade G240.00%(38/95)vs.28.57%(20/70),grade G,29.47%(28/95)vs.14.20%(10/40),higher lymph node metastasis:71.58%(68/95)vs.7.14%(5/70),worse TNM stage:stage I 15.79%(15/95)vs.34.29%(24/70),stage II 27.37%(26/95)vs.27.14%(19/70),stage II 41.05%(39/95)vs.28.57%(20/70),stage IV 15.79%(15/95)vs.10.00%(7/70),higher proportion of menopause:60.00%(57/95)vs.44.29%(31/70)and higher Ki-67 proliferation index:74.74%(71/95)vs.58.57%(41/70)(P<0.05).Lymph node metastasis(0R=2.764,P=0.038),histopathological grade G:(OR=1.942,P=0.010),TNM stage IV(OR=2.586,P<0.001),Ki-67 proliferation index greater than 14%(OR=1.376,P=0.006)were the influencing factors of high expression of ECT2 protein in breast cancer patients.The overall survival of ECT2 patients with high expression of breast cancer was even worse(P<0.05).Co

关 键 词：乳腺肿瘤 上皮细胞转化序列2 生存分析 预后 

分 类 号：R737.9[医药卫生—肿瘤]