吸烟对非小细胞肺癌患者LHX3表达水平影响及其与预后的关系  

Effect of smoking on LHx3 expression levels in postoperative non-small cell lung cancer patients and its correlationwithprognosis

作　　者：李娅[1] 王忠平[1] 梁咏雪[1] 姜霞[1] 王祝君[1] 王小培 孟鸿琼 Li Ya;Wang Zhongping;Liang Yongzue;Jiang Xia;Wang Zhujun;Wang Xiaopei;Meng Hongqiong(Department of Respiratory and Critical Care Medicine,Yan'an Hospital Affiliated to Kunming MedicalUniversity,Kunming650051,China)

机构地区：[1]昆明医科大学附属延安医院呼吸与危重症医学科,昆明650051

出　　处：《国际呼吸杂志》2023年第9期1018-1023,共6页International Journal of Respiration

基　　金：云南省科技厅科技计划项目(202001AY070001-099、2018FE001(-278))。

摘　　要：目的探究吸烟对非小细胞肺癌(NSCLC)患者LIM同源框基因3(LHX3)表达水平的影响,并分析其与患者预后的关系。方法本研究为回顾性队列研究。采用非随机抽样的方法选取2018年1月至2019年6月在昆明医科大学附属延安医院住院治疗的80例NSCLC患者为研究对象。所有患者接受肺叶切除或者全肺切除+系统性肺门纵隔淋巴结清扫术。随访患者生存情况,随访时间截至2022年12月。根据吸烟判定标准将患者分为对照组(57例)和吸烟组(23例)。通过蛋白质印迹法比较LHX3在2组癌旁组织和肿瘤组织中的表达水平。统计2组患者术后无进展生存率、总生存率、3年后出现新发病灶和新淋巴结转移的情况。采用Cox回归模型分析影响NSCLC患者术后生存的独立因素。结果2组患者肿瘤组织内LHX3表达水平均高于癌旁组织[吸烟组:(0.89±0.35)比(0.51±0.22),对照组:(0.74±0.26)比(0.49±0.17);t值分别为4.41、6.08,均P<0.001],吸烟组肿瘤组织LHX3表达水平高于对照组肿瘤组织(t=2.11,P<0.05),但2组患者癌旁组织LHX3表达水平比较差异无统计学意义(t=0.44,P>0.05)。2组患者术后3个月的无进展生存率比较,差异无统计学意义(100.00%比95.65%,χ^(2)=2.51,P>0.05),吸烟组患者术后6、18、24、36个月无进展生存率均较对照组缩短(均P<0.05)。术后1年吸烟组与对照组总生存率比较差异无统计学意义(91.30%比96.49%,χ2=0.93,P>0.05),术后2年、3年吸烟组总生存率均较对照组降低(65.22%比89.47%,52.17%比75.44%;χ^(2)值分别为6.68、4.13,均P<0.05)。术后3年,吸烟组出现新病灶转移4例,新淋巴结转移5例;对照组出现新病灶转移2例,新淋巴结转移1例,吸烟组新病灶转移和新淋巴结转移发生率均较对照组升高(均P<0.05)。多变量Cox回归分析显示,临床分期、LHX3表达、后续规范治疗和后续吸烟是影响NSCLC患者术后生存的独立因素(均P<0.05)。结论在NSCLC患者中,吸烟与肿瘤组�ObjectiveTo explore the effect of smoking on the expression of LIM-homeobox gene 3(LHX3)in postoperative patients with non-small cell lung cancer(NSCLC),and to analyze its correlation with the prognosis.MethodsThis study was a retrospective cohort study,and a non-random sampling method was conducted.A total of 80 NSCLC patients hospitalized in Yan′an Hospital Affiliated to Kunming Medical University from January 2018 to June 2019 were retrospectively recruited.All patients underwent lobectomy or total pneumonectomy plus systemic hilar mediastinal lymph node dissection.They were followed up for the survival until December 2022.Patients were allocated to control group(n=57)and smoking group(n=23)according to the criteria of smoking.The expression levels of LHX3 in NSCLC tissues and paracancerous tissues of both groups were detected by Western blot.The progression-free survival(PFS)rate,overall survival rate,and the occurrence of new lesions and lymph node metastasis at three months postoperatively in them were recorded.Independent risk factors for the postoperative survival of NSCLC patients were identified by multivariate Cox regression analysis.ResultsLHX3 was significantly upregulated in NSCLC tissues than that in paracancerous tissues of smoking group([0.89±0.35]vs[0.51±0.22],t=4.41,P<0.001)and control group([0.74±0.26]vs[0.49±0.17],t=6.08,P<0.001).The expression of LHX3 in NSCLC tissues of the smoking group was significantly higher than that of the control group(t=2.11,P<0.05).However,there was no significant difference in the expression of LHX3 in paracancerous tissues between groups(t=0.44,P>0.05).There was no difference in the PFS rate at 3 months postoperatively between groups(100.00%vs 95.65%,χ^(2)=2.51,P>0.05).PFS rate at 6,18,24,and 36 months postoperatively of smoking group was significantly shorter than that of the control group(all P<0.05).There was no significant difference in 1-year overall survival rate between the smoking group and control group(91.30%vs 96.49%,χ^(2)=0.93,P>0.05).The 2-ye

关 键 词：癌 非小细胞肺 吸烟 淋巴转移 预后 LIM同源框基因3 

分 类 号：R734.2[医药卫生—肿瘤]