基于生物信息学与相关实验探究ACLY与食管癌的关系  

Exploration of the relationship between ACLY and esophageal cancer based on bioinformatics and related experiments

作　　者：王萌 张阳阳 刘静[1] Meng WANG;Yang-Yang ZHANG;Jing LIU(Department of Gastroenterology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院消化内科,武汉430071

出　　处：《医学新知》2023年第5期334-342,共9页New Medicine

基　　金：国家自然科学基金面上项目(82072753)。

摘　　要：目的本研究以ATP-柠檬酸裂解酶(ATP citrate lyase,ACLY)为研究重点,以食管癌细胞以及在线数据库为主要研究对象,通过生物信息技术以及细胞生物实验进一步探索ACLY在食管癌细胞中的表达及其对细胞生长、增殖的影响,以期为食管癌临床诊治提供新靶点。方法利用TCGA等数据库分析ACLY在食管癌患者中的表达情况,以及ACLY在不同人群食管癌患者及不同临床分期食管癌中的表达情况。同时通过CCK-8法、EdU染色实验、平板克隆实验以及流式细胞仪等细胞基础实验,检测了ACLY变化对食管癌细胞增殖能力和细胞周期的影响。结果研究发现ACLY在包括食管癌在内的多种肿瘤组织中高表达。ACLY的表达与人种及性别无关,具有普遍性。ACLY的表达与食管癌的分期及淋巴结转移相关。进一步通过细胞生物学实验,研究证实了敲低ACLY可抑制食管癌细胞的增殖能力且可使细胞周期阻滞,过表达ACLY可提高食管癌细胞的增殖能力且可促进细胞周期进展。结论ACLY分子可影响食管癌细胞的增殖能力,ACLY可能是食管癌治疗及预后的潜在靶标。Objective This study focuses on ATP citrate lyase(ACLY),takes esophageal cancer cells and online databases as the main research subjects,and further explores the expression of ACLY in esophageal cancer cells and its effects on cell growth and proliferation through bioinformatics analysis and cell biology experiments,in order to provide a new target for clinical diagnosis and treatment of esophageal cancer.Method The databases such as TCGA were used to analyze the expression of ACLY in esophageal cancer patients,as well as the expression of ACLY in esophageal cancer patients of different race groups and different clinical stages of esophageal cancer.At the same time,the effects of ACLY changes on the proliferation and cell cycle of esophageal cancer cells were detected by basic cell experiments such as CCK-8 method,EdU staining experiment,plate cloning experiment and flow cytometry.Results ACLY is highly expressed in various tumor tissues including esophageal cancer.The expression of ACLY is universal,independent of race and gender,and its expression is related to the staging of esophageal cancer and lymph node metastasis.Further experiments in cell biology confirmed that knocking down ACLY could inhibit the proliferation of esophageal cancer cells and block the cell cycle.Overexpression of ACLY could increase the proliferative capacity of esophageal cancer cells and promote cell cycle progression.Conclusion ACLY can affect the proliferation ability of esophageal cancer cells,and ACLY may be a potential target for the treatment and prognosis of esophageal cancer.

关 键 词：食管癌 ATP-柠檬酸裂解酶 生物信息学 细胞增殖 

分 类 号：R735.1[医药卫生—肿瘤]