微波辅助双水相提取百合多糖的工艺优化及其结构表征  

Optimization of Microwave Assisted Aqueous Two Phase Extraction of Lily Polysaccharides and Its Structure Characterization

作　　者：田艳花 杨兆艳 罗爱国[2] 张玲 张立伟[3] TIAN Yanhua;YANG Zhaoyan;LUO Aiguo;ZHANG Ling;ZHANG Liwei(Food Engineering Department of Shanxi Pharmaceutical Vocational College,Taiyuan 030031,China;Department of Biological Science and Technology,Jinzhong College,Jinzhong 030619,China;Institute of Molecular Science,Shanxi University,Key Laboratory of Chemical Biology and Molecular Engineering,Ministry of Education,Taiyuan 030006,China)

机构地区：[1]山西药科职业学院食品工程系,山西太原030031 [2]晋中学院生物科学与技术系,山西晋中030619 [3]山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《食品工业科技》2023年第21期227-233,共7页Science and Technology of Food Industry

基　　金：山西省高等学校科技创新项目(2020L0770)。

摘　　要：为高效获取百合多糖和表征多糖结构,本文采用微波辅助双水相提取百合多糖,通过单因素实验和正交试验确定最佳的提取工艺;依次通过DEAE-52和Sephadex G-100柱色谱法纯化百合多糖粗提物,获得单一多糖馏分(LP2-SG),同时利用高效凝胶渗透色谱和气相色谱法分别测定LP2-SG的分子量和单糖组成,并对其结构进行初步表征。结果表明微波辅助双水相提取百合多糖的最优工艺为:微波功率400 W、硫酸铵质量分数20%和乙醇体积分数50%和料液比1:30 g/mL,百合多糖得率为10.15%±0.11%。纯化后的多糖馏分(LP2-SG)分子量为530.51 kDa,单糖组成为甘露糖、葡萄糖和半乳糖组成,其摩尔比为11.63:29.85:8.46。LP2-SG在260 nm和280 nm处无特征吸收,并具有典型的多糖红外光谱吸收特性。本研究结果为百合多糖的高效提取和深度开发提供重要参考。To obtain lily polysaccharides efficiently and characterize the structure of polysaccharides,the polysaccharides from lily was extracted by using microwave-assisted aqueous two-phase extraction in this paper.The best extraction process was determined by single factor experiment and orthogonal experiment.The crude extract of lily polysaccharides was purified via DEAE-52 and Sephadex G-100 column chromatography in turn to obtain a single fraction(LP2-SG).The molecular weight and monosaccharide composition of LP2-SG were determined by high performance gel permeation chromatography and gas chromatography,respectively,and then its structure was preliminarily characterized.The results showed that the optimum extracting parameters to achieve the highest yield of polysaccharides 10.15%±0.11%from lily by microwave-assisted aqueous two-phase extraction was obtained under the microwave power of 400 W,ammonium sulfate mass fraction of 20%,ethanol volume fraction of 50%,and solid-to-liquid ratio of 1:30 g/mL.LP2-SG with a molecular weight of 530.51 kDa was composed of mannose,glucose,and galactose with a molar ratio of 11.63:29.85:8.46.LP2-SG has no characteristic absorption at 260 nm and 280 nm,and it had typical infrared absorption characteristics of polysaccharides.The results of this study provide an important reference for the efficient extraction and deep development of lily polysaccharides.

关 键 词：微波辅助双水相提取 百合多糖 提取工艺 纯化 结构 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]