TRIM32在皮肤黑色素瘤中的表达及预后相关性分析  

Expression of TRIM32 in cutaneous malignant melanoma and its correlation with prognosis

作　　者：赵丹阳 曹东升[1] 徐结苟 谢娟[1] Zhao Danyang;Cao Dongsheng;Xu Jiegou;Xie Juan(Dept of Plastic Surgery,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601;Dept of Immunology,School of Basic Medicine,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学第二附属医院整形外科,合肥230601 [2]安徽医科大学基础医学院免疫学教研室,合肥230032

出　　处：《安徽医科大学学报》2023年第9期1551-1556,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽医科大学校科研基金(编号:2020xkj201);安徽省重点研究与开发计划项目(编号:202004a07020036)。

摘　　要：目的探讨TRIM32在皮肤黑色素瘤(CMM)中的表达及其临床病理价值。方法应用实时荧光定量多聚核苷酸链式反应(RT-PCR)检测TRIM32在人黑色素瘤细胞系(A375,MV3)以及人正常皮肤细胞系(HACAT)中的表达情况;回顾性分析31例CMM患者,应用免疫组化方法检测TRIM32在CMM患者癌组织和癌旁组织中的表达,统计患者的临床资料,分析TRIM32表达与临床特征的相关性,采用Kaplan-Meier生存分析和Cox回归模型进行预后分析。结果与HACAT正常皮肤细胞相比,TRIM32 mRNA表达水平在A375黑色素瘤细胞中明显升高(P<0.001),在MV3黑色素瘤细胞中无明显差异(P>0.05)。CMM癌组织中TRIM32蛋白表达高于癌旁组织,差异有统计学意义(P<0.001)。CMM患者中TRIM32的表达水平与肿瘤Breslow厚度(r=0.771,P<0.001)、Clark分级呈显著的正相关(r=0.635,P<0.001),与患者的性别、有无溃疡、年龄、肿瘤原发部位均无明显相关性(P>0.05)。TRIM32表达水平、肿瘤Breslow厚度、Clark分级与CMM患者生存时间相关(P<0.01),建立COX回归模型,TRIM32表达水平与CMM患者预后关联显著(P<0.05)。结论TRIM32在A375黑色素瘤细胞和CMM患者癌组织中呈高表达。TRIM32表达水平是影响CMM患者预后的独立风险因素,TRIM32可作为CMM预后判断的参考指标。Objectives To investigate the expression and clinical significance of TRIM32 in Cutaneous Malignant Melanoma patients.Methods Real-time PCR was used to detect the expression of TRIM32 in human melanoma cell lines(A375,MV3)and human normal skin cell lines(HACAT).Clinical characteristics of 31 CMM patients were collected.The expression of TRIM32 in the CMM tissues and para-cancerous tissues was detected by immunohistochemistry(IHC).The correlation between TRIM32 expression and clinical characteristics was analyzed.Kaplan-Meier survival analysis and Cox univariate analysis model were used to analyze the prognostic factors.Results The expression of TRIM32 mRNA in A375 melanoma cells was significantly higher than that in HACAT normal skin cells(P<0.001),while the expression in MV3 cells was comparable to that in HACAT cells.The expression of TRIM32 protein in the CMM tissues,detected by IHC,was significantly higher than that in the para-cancerous tissues(P<0.001)and positively correlated with Breslow thickness(r=0.771,P<0.001)and Clark grade(r=0.635,P<0.001),but not with sex,ulcer,age and primary location of tumor(P>0.05).Also,TRIM32 expression level was significantly correlated with prognosis of CMM.Conclusion TRIM32 is highly expressed in A375 melanoma cells and CMM tissues.TRIM32 may be helpful for the prognosis of CMM patients.

关 键 词：TRIM32 皮肤黑色素瘤 免疫组化 预后 

分 类 号：R739.5[医药卫生—肿瘤]