Identification of two pIgR-like molecules in teleost fish with opposite roles in mucosal immunity against bacterial infection  

作　　者：Haoyue Xu Zixuan Wang Zhenyu Huang Xiaoyun Chen Ruiqi Lin Yongyao Yu Zhen Xu 

机构地区：[1]Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,430072,China [2]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,China [3]Department of Aquatic Animal Medicine,College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China

出　　处：《Water Biology and Security》2023年第4期101-115,共15页水生生物与安全（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(32225050,32073001)to Z.X.;the National Natural Science Foundation of China(3210210315)to Y.Y.Y.

摘　　要：Polymeric immunoglobulin(Ig)receptor-like(pIgRL)molecules have been identified in teleost fish.However,compared to functional studies of their related genes(e.g.,mammalian CD300 family)in eliminating pathogen invasion while preserving homeostasis,the roles of pIgRL in teleost fish remain unclear.In this study,we demonstrated that a pair of pIgRL molecules in zebrafish,pIgRL3.5 and pIgRL4.2,were highly expressed in the intestine and immune cells.Moreover,we constructed an Edwardsiella piscicida infection model,which induced strong inflammatory responses in the zebrafish intestine.Interestingly,pIgRL3.5 and pIgRL4.2 exhibited opposite inducible expression patterns in response to bacterial infection,suggesting that they perform different roles.More importantly,by conducting overexpression and knockdown experiments,our findings demonstrated that zebrafish pIgRL3.5 played a protective role in host defense against E.piscicida infection by inhibiting excessive inflammatory responses.In contrast,pIgRL4.2 facilitated pathogen growth and dissemination in zebrafish intestine.Collectively,our findings are the first to demonstrate that a pair of pIgRL molecules in teleost fish play opposite roles in mucosal immune response to bacterial infection.Therefore,our results provide crucial insights into the conserved role of pIgRL molecules in immune regulatory functions throughout vertebrate evolution.

关 键 词：pIgR-like Mucosal immunity Edwardsiella piscicida Inflammatory response ZEBRAFISH 

分 类 号：Q178.1[生物学—水生生物学]