基于核心InDel标记樱桃番茄种质资源的遗传多样性分析与应用  

Genetic Diversity Analysis and Application of Cherry Tomato Germplasm based on Core InDel Markers

作　　者：张辉 李鑫 王志敏 胡俊玲 鲁晓晓 潘峰 潘春阳 苏文悦 徐毛毛 张敏 高浩冉 刘磊[2] 黄泽军[2] 王孝宣[2] 杜永臣[2] 李君明[2] 朱文莹 国艳梅[2] ZHANG Hui;LI Xin;WANG Zhimin;HU Junling;LU Xiaoxiao;PAN Feng;PAN Chunyang;SU Wenyue;XU Maomao;ZHANG Min;GAO Haoran;LIU Lei;HUANG Zejun;WANG Xiaoxuan;DU Yongchen;LI Junming;ZHU Wenying;GUO Yanmei(Qingdao Agricultural University,College of Horticulture,Qingdao 266000,Shandon,China;State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]青岛农业大学园艺学院,山东青岛266000 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081

出　　处：《中国蔬菜》2023年第10期26-36,共11页China Vegetables

基　　金：北京市种质创制和品种选育联合攻关项目(G20220628003-03);中国农业科学院科技创新工程项目(CAAS-ASTIPIVFCAAS);农业农村部园艺作物生物学与种质创新重点实验室项目。

摘　　要：在樱桃番茄育种进程中,遗传多样性丰富的材料有利于进一步培育重要目标性状的品种。利用从鲜食番茄和加工番茄重测序中挖掘和筛选出的48对核心In Del标记对110份樱桃番茄自交系和12个樱桃番茄商业种进行遗传多样性分析及DNA指纹图谱构建。结果表明,122份樱桃番茄材料田间表型呈现明显的遗传多样性;48对核心In Del标记在参试樱桃番茄材料中也表现良好的多态性,122份材料的遗传距离为0~0.886 cM,平均遗传距离为0.473 cM;聚类分析结果将122份材料划分为3个主要类群,其中110份自交系分散在不同类群中,且在粉贝贝、粉贝妮所属类群中分布最多。对利用自交系配制的420个F_1组合进行综合评价,发现10个入选组合中6个表现产量较好组合的遗传距离接近或大于平均值,表现一定的杂种优势。利用48对核心InDel标记构建了10个组合的亲本和12个商业种的清晰指纹图谱,可将上述材料完全区分。During the breeding process of Lycopersicon esculentum var.cerasiforme A.Gray,materials with rich genetic diversity are advantageous for further cultivation of varieties with important target traits.This paper performed genetic diversity and DNA fingerprinting on 12 commercial cherry tomato varieties collected from market,and 110 accessions of cherry tomato inbred lines bred for several years using 48 pairs of core InDel marker screened out from fresh-eating and processing tomato germplasm resources.The results indicated that the 122 accessions germplasm resources exhibited significant genetic diversity in field phenotype.And 48 pairs of core InDel marker also displayed good polymorphism among these cherry tomato germplasm resources.The genetic distances among the 122 germplasm resources were ranged from 0-0.886 cM.The average genetic distance was 0.473 cM.Clustering analysis had divided all germplasm resources into 3 main microbial strains,among them the selected 110 inbred lines distributed in different groups,mostly in the groups of‘Fenbeibei’and‘Fenbeini’.Comprehensive evaluation carried out on 420 F1 combinations prepared by using inbred lines had discovered that among the 10 chosen combinations,6 with better yields.Their genetic distances were close to or greater than the average value,showing significant advantages.Furthermore,using parents of the 10 combinations constructed by 48 pairs core InDel markers and clear fingerprint of the 12 commercial varieties could completely distinguish the above materials.

关 键 词：樱桃番茄 INDEL 遗传多样性 应用 

分 类 号：S641.2[农业科学—蔬菜学]