硫酸锌对人体肠道菌群的体外影响  

Effect of Zinc Sulfate on Human Gut Microbiota in vitro

作　　者：赵昌会 陈莫林 陈华海 胡云霏 李百元 曹林艳 尹业师 ZHAO Changhui;CHEN Molin;CHEN Huahai;HU Yunfei;LI Baiyuan;CAO Linyan;YIN Yeshi(Key Laboratory of Hunan Province for Comprehensive Utilization of Advantage Plants Resources in Hunan South,Hunan Engineering Research Center for Research and Development of Plant Resources in Nanling Area,College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou,425199)

机构地区：[1]湖南科技学院化学与生物工程学院,湘南优势植物资源综合利用湖南省重点实验室,湖南南岭地区植物资源研究开发湖南省工程研究中心,永州425199

出　　处：《基因组学与应用生物学》2023年第8期856-865,共10页Genomics and Applied Biology

基　　金：国家自然科学基金区域创新发展联合基金重点支持项目(U21A20411);湖南省自然科学基金项目(2020JJ4324)共同资助。

摘　　要：研究硫酸锌对人体肠道菌群结构及代谢产物的体外影响,为锌盐的临床应用提供肠道菌群方面的理论依据。借助体外发酵、宏基因组测序及气相色谱技术研究硫酸锌对肠道菌群及短链脂肪酸的影响。结果发现,硫酸锌显著提高克雷伯氏菌属(Klebsiella)、欧文氏菌属(Erwinia)、慢生根瘤菌属(Bradyrhizobium)、丙酸杆菌属(Propionibacterium)、担子菌门(Basidiomycota)和拉恩氏菌属(Rahnella)等的相对丰度,显著降低巨单胞菌属(Megamonas)、新月形单胞菌科(Selenomonadaceae)和厚壁菌纲(Negativicutes)等的相对丰度。种水平上硫酸锌显著提高肺炎克雷伯氏菌(Klebsiella pneumonia)、长双歧杆菌(Bifidobacterium longum)、Scandinavium goeteborgense、类肺炎克雷伯氏菌(Klebsiella quasipneumoniae)、产气克雷伯菌(Klebsiella aerogenes)、马氏棒杆菌(Corynebacterium matruchotii)、人葡萄球菌(Staphylococcus hominis)和丙酸蛛网菌(Arachnia propionica)等的相对丰度,显著降低单形巨单胞菌(Megamonas funiformis)、溃蚀齿密螺旋体(Treponema phagedenis)和超巨巨单胞菌(Megamonas hypermegale)等的相对丰度。另外,硫酸锌对古菌、真菌及病毒的丰度也有一定的影响,并影响到肠道菌群的耐药基因和短链脂肪酸含量。The effects of zinc sulfate on the structure and metabolites of human gut microbiota in vitro was investigated to provide theoretical basis for clinical application of zinc salt in gut microbiota.The effects of zinc sulfate on gut microbiota and short-chain fatty acids(SCFAs)were studied by in vitro fermentation,metagenomic sequencing and gas chromatography.The results indicated that zinc sulfate significantly increased the relative abundance of Klebsiella,Erwinia,Bradyrhizobium,Propionibacterium,Basidiomycota,Rahnella,and reduced the relative abundance of Megamonas,Selenomonadaceae and Negativicutes.At the species level,zinc sulfate significantly increased the relative abundance of Klebsiella pneumoniae,Bifidobacterium longum,Scandinavium goeteborgense,Klebsiella quasipneumoniae,Klebsiella aerogenes,Corynebacterium matruchoti,Staphylococcus hominis,Arachnia propionica,and reduced the relative abundance of Megamonas funiformis,Treponema phagedenis and Megamonas hypermegale.In addition,zinc sulfate also effected the abundance of archaea,fungi,viruses,and changed the drug resistance genes of gut microbiota and contents of short-chain fatty acids.

关 键 词：硫酸锌 人体肠道菌群 体外发酵 宏基因组测序 

分 类 号：R371[医药卫生—病原生物学]