lncRNA FEZF1-AS1和IGF2BP1在肝癌组织中的表达及临床意义  

Expression and clinical significance of lncRNA FEZF1-AS1 and IGF2BP1 in liver cancer

作　　者：姚顺春 张永川[2] YAO Shun-chun;ZHANG Yong-chuan(Department of General Surgery,Mianyang Tumor Hospital,Mianyang Sichuan 621000;Department of Hepatobiliary Surgery,The Third People′s Hospital of Mianyang,Mianyang Sichuan 621000,China)

机构地区：[1]四川省绵阳市肿瘤医院普通外科,621000 [2]四川省绵阳市第三人民医院肝胆外科,621000

出　　处：《蚌埠医学院学报》2023年第10期1346-1350,共5页Journal of Bengbu Medical College

摘　　要：目的:探讨长链非编码RNA(lncRNA)Fez家族锌指蛋白1反义RNA1(FEZF1-AS1)和胰岛素样生长因子2-mRNA结合蛋白1(IGF2BP1)在肝癌组织中的表达及临床意义。方法:收集行手术治疗的60例原发性肝癌病人的肝癌组织和癌旁组织标本,采用实时荧光定量PCR(qRT-PCR)法检测肝癌组织、癌旁组织中lncRNA FEZF1-AS1、IGF2BP1 mRNA表达水平;免疫组织化学染色法检测肝癌组织、癌旁组织中IGF2BP1蛋白表达水平;分析肝癌组织lncRNA FEZF1-AS1、IGF2BP1表达水平与临床病理特征的关系;Kaplan-Meier生存分析肝癌组织lncRNA FEZF1-AS1、IGF2BP1表达与肝癌病人生存率的关系;Pearson分析肝癌组织lncRNA FEZF1-AS1与IGF2BP1 mRNA表达水平相关性。结果:肝癌组织中lncRNA FEZF1-AS1、IGF2BP1 mRNA表达水平及IGF2BP1蛋白阳性表达率显著高于癌旁组织(P<0.01);肝癌组织中lncRNA FEZF1-AS1与IGF2BP1 mRNA表达水平呈正相关(r=0.602,P<0.01);不同年龄、性别、肿瘤大小、有无肝硬化、乙肝抗原阴阳性水平间lncRNA FEZF1-AS1、IGF2BP1表达差异无统计学意义(P>0.05),临床分期为Ⅲ~Ⅳ期、低中分化程度和有淋巴结转移的病人lncRNA FEZF1-AS1和IGF2BP1表达水平较高(P<0.05);Kaplan-Meier生存分析显示,lncRNA FEZF1-AS1高表达组病人3年累积生存率显著低于lncRNA FEZF1-AS1低表达组(31.45%vs 61.40%,P<0.01),IGF2BP1阳性表达组病人3年累积生存率显著低于IGF2BP1阴性表达组(31.18%vs 58.25%,P<0.01)。结论:肝癌组织中lncRNA FEZF1-AS1、IGF2BP1高表达,且二者表达与肝癌病人病理特征和预后有关,可能作为肝癌病人潜在的预后标志物。Objective:To investigate the expression and clinical significance of long non-coding RNA Fez family zinc finger protein 1 antisense RNA1(lncRNA FEZF1-AS1)and insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1)in liver cancer tissues.Methods:The liver cancer tissue and para-cancerous tissue specimens were collected from 60 patients with primary liver cancer.The expression of lncRNA FEZF1-AS1 and IGF2BP1 at mRNA levels in liver cancer tissues and adjacent tissues was detected with real-time fluorescent quantitative PCR(qRT-PCR)method,the expression of IGF2BP1 at protein level was detected with immunohistochemical staining method,the relationship between the expression levels of lncRNA FEZF1-AS1 and IGF2BP1 in liver cancer tissues and the clinicopathological characteristics were analyzed,the relationship between the expressions of lncRNA FEZF1-AS1 and IGF2BP1 in liver cancer tissues and the survival rate of liver cancer patients were analyzed with Kaplan-Meier survival analysis,and the correlation between lncRNA FEZF1-AS1 and IGF2BP1 mRNA expression levels in liver cancer tissues was analyzed with Pearson method.Results:The expression of lncRNA FEZF1-AS1,IGF2BP1 at mRNA level and the positive expression rate of IGF2BP1 protein in liver cancer tissues were significantly higher than those in adjacent tissues(P<0.01),and the expression of lncRNA FEZF1-AS1 and IGF2BP1 at mRNA level in liver cancer tissues were positively correlated(r=0.602,P<0.01).There was no significant differences in the expressions of lncRNA FEZF1-AS1 and IGF2BP1 among different ages,genders,tumor sizes,cirrhosis or not,and hepatitis B antigen negative and positive levels(P>0.05).The expression levels of lncRNA FEZF1-AS1 and IGF2BP1 were higher in patients with clinical stageⅢ-Ⅳ,low and moderate differentiation and lymph node metastasis(P<0.05).Kaplan-Meier survival analysis showed that the 3-year cumulative survival rate of patients in lncRNA FEZF1-AS1 high expression group was significantly lower than that in lncRNA FEZF1-AS1 low expr

关 键 词：肝肿瘤 长链非编码RNA Fez家族锌指蛋白1反义RNA1 胰岛素样生长因子2-mRNA结合蛋白1 

分 类 号：R735.7[医药卫生—肿瘤]