丙泊酚对皮下注射氯喹所致瘙痒大鼠脊髓TRPV1和CB1受体的影响  

Propofol relieves the subcutaneous chloroquine-induced pruritus in rats via TRPV1 and CB1 receptors of spinal cord

作　　者：衡冰冰 杨丹峰[1,2] 戴舒阳 上官王宁 HENG Bingbing;YANG Danfeng;DAI Shuyang;SHANGGUAN Wangning(Department of Anesthesiology and Perioperative Medicine,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou 325000,Zhejiang,China;Department of Anesthesiology,College of Medicine,Zhejiang University,Sir Run Run Shaw Hospital,Hangzhou 310016,Zhejiang,China;Department of Anesthesiology,Ruian People's Hospital,Wenzhou 325200,Zhejiang,China)

机构地区：[1]温州医科大学附属第二医院麻醉与围术期医学科,浙江温州325000 [2]浙江大学附属邵逸夫医院麻醉科,浙江杭州310016 [3]瑞安市人民医院麻醉科,浙江温州325200

出　　处：《中国临床药理学与治疗学》2023年第10期1101-1108,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省卫生厅课题资助(2022KY208)。

摘　　要：目的:探讨丙泊酚缓解大鼠皮下注射氯喹所致瘙痒的可能机制。方法:建立SD大鼠氯喹瘙痒模型并确定给药时间;18只皮下注射氯喹所致瘙痒模型成功的大鼠随机分为NS组、I组、P组,分别于皮下注射氯喹5 min后经颈内静脉导管注入生理盐水80μL/kg、脂肪乳剂80μL/kg、丙泊酚0.8 mg/kg。另随机抽取6只大鼠设为C组,于颈背部皮下和颈内静脉导管注射与其余3组同等体积生理盐水。在颈内静脉注射相应药物16 min后处死,用Western blot检测脊髓中香草素受体亚型1(TRPV1)以及大麻素受体1(CB1)受体的表达情况。结果:与NS组和I组相比,P组大鼠脊髓中TRPV1受体表达含量明显升高,差异有统计学意义(P<0.01),而C组、NS组、I组之间差异无统计学意义;CB1受体表达水平高于C组、NS组及I组,差异有统计学意义(P<0.05),而C组、NS组、I组之间差异无统计学意义。结论:丙泊酚可明显缓解大鼠皮下注射氯喹所致瘙痒,其可能通过增加大鼠脊髓中TRPV1以及CB1受体表达发挥止痒作用。AIM:To explore the possible mechanism of propofol in alleviating pruritus induced by subcutaneous injection of chloroquine in rats.METHODS:The pruritus model of chloroquine in SD rats was established and the administration time was determined.18 rats with successful pruritus model induced by subcutaneous injection of chloroquine were randomly divided into NS group,I group and P group.Normal saline 80μL/kg,fat emulsion 80μL/kg and propofol 0.8 mg/kg were injected through internal jugular vein catheter 5 minutes after subcutaneous injection of chloroquine.Another 6 rats were randomly selected as group C,and the same volume of normal saline as the other 3 groups was injected subcutaneously in the back of the neck and through the internal jugular vein catheter.The rats were killed 16 minutes after the corresponding drugs were injected into the internal jugular vein.The expressions of TRPV1 and CB1 receptors in the spinal cord were detected by Western blot.RESULTS:Compared with NS group and I group,the expression level of TRPV1 receptor in the spinal cord of P group rats was significantly increased(P<0.01),while there was no statistically significant difference between C group,NS group,and I group;The expression level of CB1 receptor was significantly higher than that of group C,NS,and I(P<0.05),while there was no statistically significant difference between group C,NS,and I.CONCLUSION:Propofol can significantly alleviate pruritus caused by subcutaneous injection of chloroquine in rats,which may exert an antipruritic effect by increasing the expression of TRPV1 and CB1 receptors in the spinal cord of rats.

关 键 词：瘙痒 大鼠 氯喹 丙泊酚 TRPV1 CB1 

分 类 号：R614[医药卫生—麻醉学] R965.2[医药卫生—外科学]