Pomolic acid and its glucopyranose ester promote apoptosis through autophagy in HT-29 colon cancer cells  

作　　者：Li-Yan Liu Teng-Hua Yu Tie-Song Liao Peng Xu Ying Wang Min Shi Bin Li 

机构地区：[1]Workstation of Academician,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [2]Department of Pharmacy,Jiangxi Cancer Hospital and Jiangxi Cancer Center,Nanchang 330029,Jiangxi Province,China [3]Department of Breast Surgery,Jiangxi Cancer Hospital and Jiangxi Cancer Center,Nanchang 330029,Jiangxi Province,China [4]Laboratory Animal Science and Technology Center,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [5]Department of Pharmacy,Shangrao Health School,Shangrao 334000,Jiangxi Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2023年第10期1756-1770,共15页世界胃肠肿瘤学杂志（英文版）（电子版）

摘　　要：BACKGROUND Colon cancer remains a leading cause of death globally.Pomolic acid(PA)can be separated from the ethyl acetate fraction of achyrocline satureioides.AIM To determine the effects of PA and its glucopyranose ester,pomolic acid-28-O-β-D-glucopyranosyl ester(PAO),on colon cancer HT-29 cells.METHODS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to measure cell viability.Apoptosis was detected via hoechst 33342 staining.PI single staining was identified by flow cytometry to determine the cycle and scratch assay was used to observe the migration of HT-29 cells.The levels of mRNA and proteins were evaluated by q polymerase chain reaction and western blotting,respectively.RESULTS PA and PAO considerably inhibited the growth of the HT-29 cell line in a time and dose-dependent manner.After the administration of PA and PAO for 24 and 48 h,cell apoptosis was significantly promoted and HT-29 cells were arrested in the G0/G1 stage.The Bax/Bcl2 ratio was also increased,which activated cysteinyl aspartate specific proteinase 3,leading to apoptosis;it also increased the expression of light chain 3 II/I and Beclin1,which activated autophagy and caused cell death.This in turn increased the expression of p62 to promote cell apoptosis,inhibiting the levels of signal transducer and activator of transcription 3(STAT3)and p-STAT3,suppressing the level of Bcl2,and promoting cell.CONCLUSION Both PA and PAO provide novel therapeutic strategies for treating colorectal cancer.

关 键 词：Colon cancer Achyrocline satureioides Pomolic acid Pomolic acid-28-O-β-D-glucopyranose 

分 类 号：R735.35[医药卫生—肿瘤]