Gene polymorphisms associated with sudden decreases in heart rate during extensive peritoneal lavage with distilled water after gastrectomy  

作　　者：Shuang Yao Yan Yuan Jun Zhang Yang Yu Guang-Hua Luo 

机构地区：[1]Clinical Medical Research Center,The Third Affiliated Hospital of Soochow University,Changzhou 213000,Jiangsu Province,China [2]Department of Anesthesiology,The Third Affiliated Hospital of Soochow University,Changzhou 213000,Jiangsu Province,China

出　　处：《World Journal of Gastrointestinal Surgery》2023年第10期2154-2170,共17页世界胃肠外科杂志（英文版）（电子版）

基　　金：Changzhou Sci and Tech Program,No.CJ20210113;Changzhou Health Young Talents Plan,No.CZQM2021006.

摘　　要：BACKGROUND Our previous study found that the telomerase-associated protein 1(TEP1,rs938886 and rs1713449)and homo sapiens RecQ like helicase 5(RECQL5,rs820196)single nucleotide polymorphisms(SNPs)were associated with changes in heart rate(HR)≥30%during peritoneal lavage with distilled water after gastrectomy.This study established a single tube method for detecting these three SNPs using two-dimensional(2D)polymerase chain reaction(PCR),and investigated whether SNP-SNP and SNP-environment interactions increase the risk of high HR variability(HRV).AIM To investigate whether genotypes,genetic patterns,SNP-SNP and SNP-environment interactions were associated with HRV.METHODS 2D PCR was used to establish a single-tube method to detect TEP1 rs938886 and rs1713449 and RECQL5 rs820196,and the results were compared with those of sanger sequencing.After adjusting for confounders such as age,sex,smoking,hypertension,and thyroid dysfunction,a nonconditional logistic regression model was used to assess the associations between the genotypes and the genetic patterns(codominant,dominant,overdominant,recessive,and additive)of the three SNPs and a risk≥15%or≥30%of a sudden drop in HR during postoperative peritoneal lavage in patients with gastric cancer.Gene-gene and geneenvironment interactions were analyzed using generalized multifactor dimensionality reduction.RESULTS The coincidence rate between the 2D PCR and sequencing was 100%.When the HRV cutoff value was 15%,the patients with the RECQL5(rs820196)TC genotype had a higher risk of high HRV than those who had the TT genotype(odds ratio=1.97;95%CI:1.05-3.70;P=0.045).Under the codominant and overdominant models,the TC genotype of RECQL5(rs820196)was associated with a higher risk of HR decrease relative to the TT and TT+CC genotypes(P=0.031 and 0.016,respectively).When the HRV cutoff value was 30%,patients carrying the GC-TC genotypes of rs938886 and rs820196 showed a higher HRV risk when compared with the GG–TT genotype carriers(P=0.01).In the three-factor model of rs

关 键 词：Homo sapiens RecQ like helicase 5 Telomerase-associated protein 1 Polymorphism Peritoneal lavage Heart rate variability Two-dimensional polymerase chain reaction 

分 类 号：R735.2[医药卫生—肿瘤]