Sprouty2蛋白调节去势抵抗性前列腺癌细胞增殖、迁移和凋亡的机制研究  

Mechanism of Sprouty2 Protein Regulation on Proliferation,Migration and Apoptosis of Castrated Resistant Prostate Cancer Cells

作　　者：姜涵中 王立森 孙立江[3] JIANG Hanzhong;WANG Lisen;SUN Lijiang(School of Medicine,Qingdao University,Qingdao 266071,China;Laizhou Traditional Chinese Medicine Hospital,Yantai 261400,China;Affiliated Hospital of Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学医学院,山东青岛266071 [2]莱州市中医院,山东烟台261400 [3]青岛大学附属医院,山东青岛266071

出　　处：《北华大学学报（自然科学版）》2023年第6期754-758,共5页Journal of Beihua University（Natural Science）

基　　金：山东省科技厅医药卫生科技发展计划项目(2021310729)。

摘　　要：目的 探讨快速发育生长因子同源蛋白2抗体(Sprouty2)蛋白调节去势抵抗性前列腺癌细胞增殖、迁移和凋亡的机制.方法 根据试验目的将人去势抵抗性前列腺癌细胞分为Sprouty2蛋白高表达组、阴性对照组、空白对照组.Sprouty2蛋白高表达组通过siRNA技术将小激活RNA(saRNA)转入人去势抵抗性前列腺癌细胞中进行干预;阴性对照组通过siRNA技术将空载体质粒转入人去势抵抗性前列腺癌细胞中进行干预;空白对照组进行常规培养;取对数生长期细胞用于后续实验.噻唑蓝(MTT)测定细胞增殖情况;划痕实验测定细胞迁移能力;Annexin V-FITC和PI双染色测定细胞凋亡情况.Western blot测定Myc促癌基因(c-Myc)、SRY盒转录因子4(Sox4)、E-box结合同源盒1(ZEB1)蛋白、E-钙黏蛋白、波形蛋白表达.结果 Sprouty2蛋白免疫细胞百分比、Sprouty2蛋白免疫细胞荧光强度在各组间比较差异均具有统计学意义(P<0.05).细胞增殖活力在阴性对照组与空白对照组之间比较差异无统计学意义(P>0.05);Sprouty2蛋白高表达组细胞迁移率明显低于阴性对照组和空白对照组,细胞凋亡率明显高于阴性对照组和空白对照组(P<0.01);细胞迁移率和细胞凋亡率在阴性对照组与空白对照组之间比较差异无统计学意义(P>0.05).E-钙黏蛋白表达水平明显高于阴性对照组和空白对照组(P<0.01);c-Myc、Sox4、ZEB1蛋白、E-钙黏蛋白、波形蛋白表达水平在阴性对照组与空白对照组之间比较差异无统计学意义(P>0.05).结论 Sprouty2蛋白高表达可能通过下调c-Myc、Sox4、ZEB1蛋白、波形蛋白表达,上调Sprouty2蛋白表达抑制去势抵抗性前列腺癌细胞的增殖、迁移和凋亡.Objective To investigate the mechanism of rapid growth factor homologous protein 2 antibody(Sprouty2)protein regulating the proliferation,migration and apoptosis of castrated resistant prostate cancer cells.Method The human castration-resistant prostate cancer cells were divided into three groups based on the experimental purpose,the Sprouty2 protein high-expression group,the negative control group,and the blank control group.In the Sprouty2 protein high-expression group,small activating RNA(saRNA)was transfected into the human castration-resistant prostate cancer cells by using siRNA technology for intervention.In the negative control group,empty vector plasmids were transfected into the human castration-resistant prostate cancer cells by using siRNA technology for intervention.The blank control group was cultured conventionally.Logarithmic growth phase cells were used for subsequent experiments.The MTT assay was used to determine cell proliferation.The scratch assay was used to determine cell migration ability.Annexin V-FITC and PI double staining were used to determine cell apoptosis.Western blot was used to determine the expression of Myc oncogene(c-Myc),SRY box transcription factor 4(Sox4),E-box binding homologous box 1(ZEB1)protein,E-cadherin and actin.Results The percentage of Sprouty2 protein immune cells and the fluorescence intensity of Sprouty2 protein immune cells showed statistically significant differences among the groups(P<0.05).There was no statistically significant difference in cell proliferation activity between the negative control group and the blank control group(P>0.05).The cell migration rate of Sprouty2 protein high-expression group was significantly lower than that of negative control group and blank control group,and the apoptosis rate was significantly higher than that of negative control group and blank control group(P<0.01);There was no significant difference in cell migration rate and apoptosis rate between negative control group and blank control group(P>0.05).The expression level

关 键 词：前列腺癌 快速发育生长因子同源蛋白2抗体蛋白 细胞增殖 细胞迁移 细胞凋亡 机制 

分 类 号：R737[医药卫生—肿瘤]