Fruit size control by a zinc finger protein regulating pericarp cell size in tomato  

作　　者：Fangfang Zhao Jiajing Zhang Lin Weng Meng Li Quanhua Wang Han Xiao 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China [2]University of Chinese Academy of Sciences,No.19(A)Yuquan Road,Shijingshan District,Beijing 100049,China [3]Life and Environment Science College,Shanghai Normal University,No.100 Guilin Rd,Shanghai 200234,China

出　　处：《Molecular Horticulture》2021年第1期124-139,共16页分子园艺（英文）

基　　金：The work was supported by National Key Research and Development Program of China(2016YFD0101900);the National Natural Science Foundation of China(31672164);Agriculture and Rural Affairs Commission of Shanghai municipality(Agriculture Applied Technology Development Program,HuNongKeGong Grant No.2015-6-1-7).

摘　　要：Fruit size is largely defined by the number and size of cells in the fruit.Endoreduplication–a specialized cell cycle–is highly associated with cell expansion during tomato fruit growth.However,how endoreduplication coupled with cell size is regulated remains poorly understood.In this study,we identified a zinc finger gene SlPZF1(Solanum lycopersicum PERICARP-ASSOCIATED ZINC FINGER PROTEIN 1)that was highly expressed in the pericarp of developing fruits.Plants with altered SlPZF1 expression produced smaller fruits due to the reduction in cell size associated with weakened endoreduplication.Overexpressing SlPZF1 delayed cell division phase by enhancing early expression of several key cell cycle regulators including SlCYCD3;1 and two plant specific mitotic cyclin-dependent protein kinase(SlCDKB1 and SlCDKB2)in the pericarp tissue.Furthermore,we identified 14 putative SlPZF1 interacting proteins(PZFIs)via yeast two hybrid screening.Several PZFIs,including Pre-mRNA-splicing factor(SlSMP1/PZFI4),PAPA-1-like conserved region family protein(PZFI6),Fanconi anemia complex components(PZFI3 and PZFI10)and bHLH transcription factor LONESOME HIGHWAY(SILHW/PZFI14),are putatively involved in cell cycle regulation.Our results demonstrate that fruit growth in tomato requires balanced expression of the novel cell size regulator SlPZF1.

关 键 词：Zinc finger protein ENDOREDUPLICATION Cell cycle Cell size Fruit size Tomato(Solanum lycopersicum) 

分 类 号：R73[医药卫生—肿瘤]