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作 者:吴明阳 赵思毅 杨小丽 李益 高龙梅 范香全 WU Mingyang;ZHAO Siyi;YANG Xiaoli;LI Yi;GAO Longmei;FAN Xiangquan(Dazhou Academy of Agricultural Sciences,Dazhou Sichuan 635000)
出 处:《现代农业科技》2023年第20期34-37,共4页Modern Agricultural Science and Technology
基 金:四川省农业科技成果转化资金项目(2021NZZJ0014);国家现代农业产业技术体系四川薯类创新团队项目(川财教〔2019〕59号);四川省“十四五”农作物及畜禽育种攻关项目(2021YFYZ0019)。
摘 要:本试验以马铃薯新品种达芋6号为试验材料,采用正交试验方法,研究培养基中不同种类、不同浓度激素对达芋6号马铃薯茎尖培养的影响。结果表明:NAA 0.05 mg/L+6-BA 0.5 mg/L+GA_(3)0.1 mg/L的处理成苗率、苗高优于其余处理;影响达芋6号成苗率及苗高的因素重要性排序为NAA浓度>GA3浓度>6-BA浓度;最有利于达芋6号成苗的激素水平为NAA 0.05 mg/L、GA_(3)0.1 mg/L、6-BA 1.0 mg/L;最有利于达芋6号苗高生长的激素水平为NAA 0.05 mg/L、GA_(3)0.1 mg/L、6-BA 0.1 mg/L。Taking a new potato variety Dayu 6 as the experimental material,this paper studied the effects of different types and concentrations of hormones in culture medium on stem-apex meristem culture of Dayu 6 by using orthogonal experiment as method.The results showed that the seedling formation rate and seedling height of NAA 0.05 mg/L+6-BA 0.5 mg/L+GA_(3)0.1 mg/L treatment were superior to other treatments.The importance order of the factors affecting the seedling formation rate and seedling height of Dayu 6 was NAA concentration>GA_(3)concentration>6-BA concentration.The most favorable hormone levels for the seedling formation of Dayu 6 were NAA 0.05 mg/L,GA_(3)0.1 mg/L,6-BA 1.0 mg/L.The most favorable hormone levels for the seedlings height of Dayu 6 were NAA 0.05 mg/L,GA_(3)0.1 mg/L,6-BA 0.1 mg/L.
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