聚乙烯微塑料对海洋微藻生长和卤代烃释放的影响  

Effects of Polyethylene Microplastics on Growth and Halocarbon Release of Marine Microalgae

作　　者：杨帆 陈挚 翟星 杨桂朋[1,3] 何真 YANG Fan;CHEN Zhi;ZHAI Xing;YANG Gui-peng;HE Zhen(Key Laboratory of Marine Chemistry Theory and Technology,College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China;Key Laboratory of Marine Eco-Environmental Science and Technology,Ministry of Natural Resources,Qingdao 266061,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学化学化工学院,海洋化学理论与工程技术教育部重点实验室,青岛266100 [2]自然资源部海洋生态环境科学与技术重点实验室,青岛266061 [3]青岛海洋科学与技术试点国家实验室,海洋生态与环境科学功能实验室,青岛266237

出　　处：《环境科学》2023年第10期5852-5860,共9页Environmental Science

基　　金：国家自然科学基金项目(41830534);山东省自然科学基金项目(ZR2021MD034);自然资源部海洋生态环境科学与技术重点实验开放基金项目(MEEST-2021-01)。

摘　　要：挥发性卤代烃(VHCs)是一类重要的痕量温室气体和臭氧破坏物质,在全球气候变化中发挥重要作用.微藻释放VHCs受海洋环境因素的影响.微塑料作为海洋中一种重要新型污染物其对海洋微藻释放VHCs的影响研究比较少见.基于小新月菱形藻(Nitzschia closterium f.minutissima)和东海原甲藻(Prorocentrum donghaiense)的室内单种培养实验,通过测定其藻细胞密度、最大光量子效率(F_(v)/F_(m))、活性氧(ROS)和VHCs浓度,研究聚乙烯(PE)微塑料长期暴露对小新月菱形藻和东海原甲藻生长和VHCs释放的影响.结果表明,PE微塑料胁迫对小新月菱形藻生长主要表现抑制作用,而对东海原甲藻生长表现出促进作用.50μm的PE微塑料的添加对小新月菱形藻和东海原甲藻的光合作用起到遮蔽作用,导致二者的F_(v)/F_(m)受到抑制,且其对东海原甲藻的抑制作用更显著.与对照组相比,PE微塑料胁迫刺激藻细胞的ROS产生量增加,引起小新月菱形藻和东海原甲藻的氧化应激反应,从而促进两种微藻3种溴代烃的释放.Volatile halocarbons(VHCs)are important trace greenhouse gases and ozone-destroying substances and play an important role in global climate change.As an important producer of VHCs,the release of VHCs by marine microalgae is affected by marine environmental factors.Microplastics are an important pollutant in the ocean;however,there are few studies on VHCs release from marine microalgae under the influence of microplastics.This study aimed to explore the effects of different concentrations of polyethylene(PE)microplastics on the growth,photosynthesis,oxidative stress,and release of VHCs by diatoms and dinoflagellates by measuring the density of algae,maximum photoquantum efficiency(F_(v)/F_(m)),reactive oxygen species(ROS),and concentration of VHCs.The results revealed that PE microplastics mainly inhibited the growth of Nitzschia closterium f.minutissima and promoted the growth of Prorocentrum donghaiense.The addition of 50μm PE microplastics had a shielding effect on the growth of the two microalgae,resulting in the inhibition of F_(v)/F_(m)of two kinds of microalgae,and the inhibition effect of PE microplastics on P.donghaiense was more significant.Compared with that in the control group,PE microplastic stress stimulated the increase in ROS production in algal cells,which caused an oxidative stress response in these microalgae,thereby promoting the release of three types of volatile brominated halocarbons.

关 键 词：挥发性卤代烃(VHCs) 小新月菱形藻 东海原甲藻 聚乙烯 微塑料 生长抑制 

分 类 号：X171.5[环境科学与工程—环境科学]