基于EF-1α和β微管蛋白基因序列的棉花枯萎病菌遗传多样性和单倍型分析  

Genetic diversity and haplotype analysis of cotton Fusarium wilt based on gene sequences of EF-1αandβ-tubulin

作　　者：王海莹 陈小海 钟烨仪 龚举武[1] 刘平[1] Chin Yaoxian 王沛政 袁有禄[1] Wang Haiying;Chen Xiaohai;Zhong Yeyi;Gong Juwu;Liu Ping;Chin Yaoxian;Wang Peizheng;Yuan Youlu(Institute of Cotton Research of Chinese Academy of Agricultural Sciences/National Key Laboratory of Cotton Bio-breeding and Integrated Utilization,Anyang,Henan 455000,China;Key Laboratory for Coastal Marine Eco-environment Process and Carbon Sink of Hainan Province,Hainan Tropical Ocean University,Sanya,Hainan 572022,China;Yazhou Bay Innovation Institute,Hainan Tropical Ocean University,Sanya,Hainan 572022,China)

机构地区：[1]中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室,河南安阳455000 [2]海南热带海洋学院海南省近岸海洋生态环境过程与碳汇重点实验室,海南三亚572022 [3]海南热带海洋学院崖州湾创新研究院,海南三亚572022

出　　处：《棉花学报》2023年第4期334-344,共11页Cotton Science

基　　金：棉花生物学国家重点实验室开放课题(CB2021A10);国家级大学生创新创业训练计划项目(202011100029)。

摘　　要：【目的】通过分析棉花枯萎病菌的遗传多样性,探究新疆棉花枯萎病菌株的分群及其演化。【方法】2022年在新疆不同植棉区共分离出22株棉花枯萎病菌株,对延伸因子1α(elongation factor-1α,EF-1α)和β微管蛋白基因进行扩增、测序,并从美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库获取36个棉花枯萎病菌株的相关基因序列信息。基于上述基因序列分别进行系统进化分析和单倍型分析。【结果】基于57条EF-1α基因序列的进化树分析表明,棉花枯萎病菌可分为3大群,第1大群包含来自新疆、河北和澳大利亚的共31个枯萎病菌株,该大群可分成4个亚群;第2大群包含25个枯萎病菌株,构成比较复杂,可分成3个亚群;第3大群仅包含美国菌株LA140。基于28条β微管蛋白基因序列的进化树分析表明,本次分离的新疆棉花枯萎病菌株与棉花枯萎病菌7号和8号生理小种不同。根据EF-1α基因序列构建的单倍型网络将棉花枯萎病菌株分为19个单倍型,新疆21个棉花枯萎病菌株归属于有共同起源的5种单倍型。【结论】本研究分离的新疆棉花枯萎病菌株与已报道的棉花枯萎病菌1~8号生理小种均不相同,但与河北菌株的亲缘关系较近。EF-1α单倍型分析表明,本研究中的所有棉花枯萎病菌均从1号生理小种演化而来。[Objective]This research aims to characterize the grouping and evolution of cotton Fusarium oxysporium f.sp.vasinfectum(FOV)strains in Xinjiang on the basis of genetic diversity analysis.[Method]A total of twenty-two FOV strains from different cotton planting areas in Xinjiang were isolated in 2022,and EF-1αandβ-tubulin gene were amplified and sequenced.Sequences of other thirty-six cotton FOV strains were downloaded from National Center for Biotechnology Information(NCBI).Phylogenetic analysis and haplotype analysis were carried out based on the above-mentioned sequences.[Result]Phylogenetic tree analysis based on fifty-seven EF-1αgene sequences indicated that FOV strains can be divided into three groups.The first group included 31 FOV strains from Xinjiang,Hebei province and Australia.This group can be further divided into four subgroups.The second group,including 25 FOV strains and relatively complex composition,can be divided into three subgroups.The last group only included LA140 from America.Phylogenetic tree analysis based on twenty-eightβ-tubulin gene sequences showed that FOV strains from Xinjiang are different from the race 7 and race 8.All strains were divided into nineteen haplotypes by haplotype network analysis based on EF-1αsequences.Twenty-one FOV strains isolated from Xinjiang belongs to five haplotypes,which share a common origin.[Conclusion]The strains isolated from Xinjiang in this research are different from the reported race 1 to race 8,but share a relatively close relationship with FOV in Hebei province.Haplotype analysis of EF-1αindicates all FOV strains in this research are evolved from race 1.

关 键 词：棉花 枯萎病菌 单倍型分析 遗传多样性分析 延伸因子1α β微管蛋白 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]