红细胞压积对便携式血糖仪检测结果的影响及其外部校正的可行性评估  

Effects of Hematocrit on the Results of Portable Blood Glucose Meter andRelated Feasibility Evaluation for External Correction

作　　者：刘艳婷[1] 肖光军[1] 魏容[1] 梁艳丽[1] LIU Yanting;XIAO Guangjun;WEI Rong;LIANG Yanli(Department of Clinical Laboratory and Basic Laboratory,Suining Central Hospital,Suining 629000,China)

机构地区：[1]遂宁市中心医院检验科,四川遂宁629000

出　　处：《标记免疫分析与临床》2023年第8期1420-1425,共6页Labeled Immunoassays and Clinical Medicine

基　　金：遂宁市中心医院院级科研课题(编号:2021y30)。

摘　　要：目的 探讨红细胞压积(hematocrit, HCT)对便携式血糖仪检测结果的影响,并评估通过HCT值外部校正提高便携式血糖仪检测结果准确性的可行性。方法 收集70份肝素钠抗凝静脉血标本,调整其血糖水平和HCT后,分别使用3种便携式血糖仪(以仪器A、仪器B、仪器C表示)和生化分析仪检测其血糖水平,评估每台便携式血糖仪检测结果的绝对偏差或相对偏差与标本HCT值的相关性,并通过HCT值对便携式血糖仪的检测结果进行外部校正,评估其检测结果外部校正前后与生化分析仪检测结果的可比性。合格率≥95%,表示为可比性合格的仪器。结果 共65份标本纳入研究,HCT<35%时,仪器A、B、C的合格率依次为100.00%、16.67%、41.67%;35%≤HCT≤55%时,仪器A、B、C的合格率依次为100.00%、100.00%、89.47%;HCT>55%时,仪器A、B、C的合格率依次为96.92%、38.46%、53.85%。与生化分析仪的检测结果比较,仪器A的检测结果具有可比性,而仪器B和C则不具有可比性,且HCT<35%或HCT>55%时,仪器B和C检测结果的绝对偏差或相对偏差与标本HCT值存在高度相关性。HCT值外部校正前,仪器仪器A、仪器B和仪器C的总体合格率分别为96.92%、38.46%、53.85%;外部校正后,其总体合格率分别为33.85%、81.54%、76.92%,与校正前的总体合格率比较,差异均具有统计学意义(P<0.01)。结论 标本HCT值偏低或偏高将影响部分便携式血糖仪检测结果的准确性,故便携式血糖仪临床应用前应进行抗HCT干扰能力评价,并根据评价结果及其所服务人群来选择适宜的便携式血糖仪,必要时,可通过HCT值外部校正的方法来提高便携式血糖仪检测结果准确性。Objective To explore the effect of hematocrit(hematocrit,HCT)on the results of portable blood glucose meters,and to evaluate related feasibility of using HCT value to externally correct the results of portable glucose meter.Methods Seventy heparin sodium anticoagulant venous blood samples were collected for the study.After adjusting the blood glucose concentration and HCT,the blood glucose concentration was detected by three types of portable blood glucose meters respectively(represented by instrument A,instrument B,instrument C)and biochemical analyzer.We then evaluated comparabilty of test results between each of the portable blood glucose meter and biochemical analyzer,as well as correlation between absolute deviation or relative deviation of the test results and specimens HCT value.The portable blood glucose meter test results affected by HCT were corrected by HCT value.We evaluated comparabilty between correction results and biochemical analyzer test results.An instrument with qualification rate of 95%or more was considered to be comparable.Results A total of 65 specimens were included in the study,and the qualified rates of instrument A,B and C were 100%,16.67%and 41.67%,respectively,when HCT<35%;The qualified rates of instrument A,B and C were 100%,100%and 89.47%,respectively,when 35%≤HCT≤55%;The qualified rates of instrument A,B and C were 96.92%,38.46%and 53.85%,respectively,when HCT>55%.The detection results of instrument A were comparable with the detection results of biochemical analyzer,but the detection results of instrument B and C were not comparable with the detection results of biochemical analyzer.When HCT<35%or HCT>55%,the absolute or relative deviation of the detection results of instrument B and C was highly correlated with the HCT value of the specimen.Before external correction of HCT value,the overall qualified rates of instrument A,instrument B and instrument C were 96.92%,38.46%and 53.85%,respectively.After external correction,the overall qualified rates were 33.85%,81.54%and 76.92%

关 键 词：红细胞压积 便携式血糖仪 血糖 干扰 

分 类 号：R-331[医药卫生]