“标本配穴”艾灸对腹泻型肠易激综合征伴焦虑模型大鼠心率变异性、心房利钠肽的影响  

Effects of moxibustion of"biaoben acupoint combination"on heart rate variability,atrial natriuretic peptide in the model rats of IBS-D complicated with anxiety

作　　者：吴帆 李佳 王静芝 王华 吴松 卢威 WU Fan;LI Jia;WANG Jing-zhi;WANG Hual;WU Song;LU Wei(College of Acupuncture-Moxibustion,Orthopedics and Traumatology,Hubei University of CM,Wuhan 430061,China;College of Clinical Chinese Medicine,Hubei University of CM)

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430061 [2]湖北中医药大学中医临床学院

出　　处：《中国针灸》2023年第10期1139-1147,共9页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:81973931;全国名老中医药专家传承工作室建设项目:国中医药人教函[2022]75号。

摘　　要：目的:比较“标本配穴”和“常规取穴”艾灸对腹泻型肠易激综合征(IBS-D)伴焦虑模型大鼠心率变异性(HRV)和心肌心房利钠肽(ANP)表达的影响。方法:从50只健康SPF级3月龄雌性SD大鼠中随机选取8只作为空白组,剩余大鼠制备IBS-D伴焦虑模型,将造模成功的24只大鼠随机分为模型组、常规组和标本组,每组8只。常规组予“天枢”“足三里”“上巨虚”艾灸干预,标本组予“内关”“足三里”“关元”艾灸干预,每次20 min,每天1次,共干预14 d。分别于干预前后比较各组大鼠体质量、粪便含水率;应用腹壁撤退反射(AWR)实验评价内脏高敏感性,高架十字迷宫和明暗穿箱实验评价焦虑状态。干预后,比较各组大鼠HRV,采用透射电镜观察各组大鼠肠黏膜超微结构,Western blot法及免疫荧光法检测大鼠心肌组织ANP表达。结果:干预前,与空白组比较,模型组、常规组、标本组大鼠体质量、内脏疼痛阈值降低(P<0.05),粪便含水率、AWR评分(扩张压力为40、60、80 mm Hg时,1 mm Hg≈0.133 kPa)升高(P<0.05),开放臂停留时间、进入开放臂次数、总运动路程(高架十字迷宫)及明箱停留时间、明暗箱穿梭次数、总运动路程(明暗穿箱)均减少(P<0.05)。干预后,与空白组比较,模型组大鼠体质量、内脏疼痛阈值、RR间期标准差(SDNN)、连续RR间隔均方差的平方根(RMSSD)降低(P<0.05),粪便含水率、AWR评分(扩张压力为40、60、80 mm Hg时)、低频/高频(LF/HF)、ANP表达升高(P<0.05),开放臂停留时间、进入开放臂次数、总运动路程(高架十字迷宫)及明箱停留时间、明暗箱穿梭次数、总运动路程(明暗穿箱)减少(P<0.05);与模型组比较,常规组和标本组大鼠体质量、内脏疼痛阈值、SDNN、RMSSD升高(P<0.05),粪便含水率、AWR评分(扩张压力为60、80 mm Hg时)、LF/HF、ANP表达降低(P<0.05),开放臂停留时间、进入开放臂次数、总运动路程(高架十字迷宫)及�Objective To compare the effects on the heart rate variability(HRV)and the expression of the atrial natriuretic peptide(ANP)in the model rats of rritable bowel syndrome with predominant diarrhea(IBS-D)rats complicated with anxiety between moxibustion of"biaoben acupoint combination"and that of"conventional acupoint combination".Methods Of 50 healthy SPF female SD rats,aged 3 months,8 rats were selected randomly as a blank group,and the rest rats were prepared to be the model of IBS-D complicated with anxiety.Twenty-four rats after successfully modeled were randomized into a model group,a conventional acupoint combination group(convention group)and a biaoben acupoint combination group(biaoben group),8 rats in each one.In the convention group,moxibustion was delivered at"Tianshu"(ST 25),"Zusanli"(ST 36)and"Shangjuxu"(ST 37);and in the biaoben group,moxibustion was applied to"Neiguan"(PC 6),"Zusanli"(ST 36),and"Guanyuan"(CV 4).One session of moxibustion took 20 min,once daily,for 14 days in total.Before and after intervention,the body mass and fecal moisture content were compared in the rats of each group;using abdominal wall withdrawal reflex,the visceral hypersensitivity was evaluated;with elevated plus maze(EPM)and light-dark box(LDB),the anxiety conditions were assessed.After intervention,HRV was compared among groups,the ultrastructure of intestinal mucosa was observed under the transmission electron microscope in the rats of each group,and ANP expression in the myocardial tissue was detected using Western blot method and immunofluorescence.Results Before the intervention,compared with the blank group,the body mass and visceral pain threshold of rats were reduced in the model group,the convention group and the biaoben group(P<0.05),fecal moisture content and AWR scores(at the dilatation pressure of 40,60 and 80 mm Hg,1 mm Hg~0.133 kPa)were elevated(P<0.05);and time in the open arm,the open arm entry number and the total movement distance(EPM),the time spent in the light compartment,the number of dark to light t

关 键 词：腹泻型肠易激综合征 标本配穴 心肠同调 心率变异性 心房利钠肽 

分 类 号：R224.2[医药卫生—中医基础理论]