基于宏基因组学分析广西地区生水牛乳中嗜冷菌多样性  

Metagenomics analysis of diversity of psychrophilic bacteria in raw buffalo milk in Guangxi

作　　者：赵利旦 黄丽 韦盘秋 黄加祥 曾庆坤 李玲 ZHAO Li-dan;HUANG Li;WEI Pan-qiu;HUANG Jia-xiang;ZENG Qing-kun;LI Ling

机构地区：[1]广西壮族自治区水牛研究所,广西壮族自治区水牛乳质量与安全控制技术工程研究中心,广西南宁530001 [2]广西大学动物科学技术学院,广西南宁530004

出　　处：《饲料研究》2023年第19期81-86,共6页Feed Research

基　　金：国家重点研发计划资助(项目编号:2022YFD1600102);广西科技重大专项(项目编号:桂科AA22068099-10)南宁市创新创业领军人才“邕江计划”(项目编号:2020017);广西壮族自治区水牛研究所基本科研业务费项目(项目编号:水牛基2103)。

摘　　要：试验将采自广西地区不同冷藏时间(24、48 h)的散户和牧场中新鲜生水牛乳进行宏基因组测序,分析生水牛乳在存储过程中嗜冷菌多样性。结果表明,在属和种水平上,冷藏24、48 h生水牛乳主要的优势嗜冷菌组成相同,但相对丰度存在差异。主坐标分析表明,不同冷藏时间嗜冷菌在种水平上菌群结构相似。聚类分析结果表明,生水牛乳样品中嗜冷菌在属和种水平上构成在组内个体差异较大。生水牛乳中具有潜在危害的嗜冷菌包括荧光假单胞菌、霉实假单胞菌、隆德假单胞菌、韩国假单胞菌、鲍曼不动杆菌、约翰不动杆菌、Acinetobacter guillouiae和蜡样芽孢杆菌等。研究表明,试验有助于发现广西地区生水牛乳中嗜冷菌污染的现状,可为控制生水牛乳中主要嗜冷菌提供参考。Metapomic sequencing was conducted on raw buffalo milk collected from individual households and farms with different refrigeration time(24,48 h)in Guangxi,and the diversity of psychrophilic bacteria during storage was analyzed.The results showed that at the genus and species levels,composition of the dominant psychrophilic bacteria was the same in 24,48 h,but their relative abundance was different.Principal coordinate analysis showed that the microbial community structure of psychrophilic bacteria at the species level was similar at different refrigeration times.The clustering analysis results indicated that there were significant individual differences in the composition of psychrophilic bacteria at the genus and species levels in the raw buffalo milk samples within the group.The potentially harmful psychrophilic bacteria in raw buffalo milk included Pseudomonas fluorescens,Pseudomonas fragi,Pseudomonas koreensis,Acinetobacter baumannii,Acinetobacter johnsonii,Acinetobacter guillouiae,Bacillus cereus,etc.The experiment indicates that the experiment is helpful to find the current situation of psychrophilic bacteria contamination in raw buffalo milk in Guangxi,which can provide reference for the control of the main psychrophilic bacteria in raw buffalo milk.

关 键 词：冷藏时间 生水牛乳 嗜冷菌 宏基因组学 

分 类 号：S823[农业科学—畜牧学]