云南松松塔抗HIV活性提取物对人冠状病毒229E抑制活性的研究  

The Inhibitory Activity of Pinus yunnanensis Pine Extract with Anti-HIV Activity against Human Coronavirus 229E

作　　者：李亚飞 尚方建 罗春雨 石哲芳 刘奇 Li Yafei;Shang Fangjian;Luo Chunyu;Shi Zhefang;Liu Qi(Pre-clinical College,Dali University,Dali,Yunnan 671000,China;Yunnan Provincial Key Laboratory of Insect Biomedical Research and Development,Dali,Yunnan 671000,China;Baoding Hospital of Beijing Children's Hospital,Capital Medical University,Baoding,Hebei 071000,China)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000 [3]首都医科大学附属北京儿童医院保定医院,河北保定071000

出　　处：《大理大学学报》2023年第10期21-26,共6页Journal of Dali University

基　　金：国家自然科学基金项目(81660337,81703573);云南省教育厅科学研究基金项目(2022Y816)。

摘　　要：目的:评价云南松松塔抗HIV活性提取物(以下简称“松塔提取物”)对人冠状病毒(HCoV)-229E的抑制活性,初步明确其作用靶点。方法:构建HCoV-229E S蛋白介导的细胞-细胞融合模型及假病毒模型,检测松塔提取物对HCoV-229E的抑制活性;利用时间-移除实验明确作用靶点,时间-添加实验确定其作用时间。结果:松塔提取物能够抑制HCoV-229E S蛋白介导的细胞-细胞融合现象发生,半抑制浓度(IC50)为(0.136±0.010)mg/mL;能有效抑制HCoV-229E假病毒感染,IC50为(0.069±0.015)mg/mL;时间-移除实验以及时间-添加实验显示松塔提取物靶向于HCoV-229E S蛋白,作用在感染早期(1 h前);不同浓度松塔提取物对293T及Huh-7细胞无明显毒性作用。结论:松塔提取物能靶向HCoV-229E S蛋白,在感染早期抑制HCoV-229E的感染,是一种安全性高的候选药物。Objective:To evaluate the inhibitory activity of Pinus yunnanensis pine extract(referred to as"pine extract")with anti-HIV activity against human coronavirus HCoV-229E and preliminarily determine its target.Methods:A cell-cell fusion model and model mediated by HCoV-229E S protein were constructed to detect the inhibitory activity of pine extract against HCoV-229E.Time-of-addition and time-of-removal experiments were performed to determine the target and the timing of action.Results:Pine extract could inhibit the cell-cell fusion mediated by HCoV-229E S protein,with an IC50 of(0.136±0.010)mg/mL.It effectively inhibited HCoV-229E infection,with an IC50 of(0.069±0.015)mg/mL.Time-of-removal and time-of-addition experiments showed that pine extract targeted the HCoV-229E S protein and acted in the early stage of infection(1 h before);different concentrations of pine tower extracts have no significant toxicity to 293T and Huh-7 cells.Conclusion:Pine extract can target the HCoV-229E S protein and inhibit HCoV-229E infection in the early stage,making it a safe candidate drug.

关 键 词：云南松 松塔提取物 HCoV-229E 抑制活性 

分 类 号：R285.5[医药卫生—中药学]