小麦茎秆蔗糖积累转运全基因组关联分析  

Genome-wide Association Study of Stems Sucrose Accumulation and Translocation in Wheat

作　　者：窦佳欣 田甜 王鹏[1] 刘媛[1] 陈涛 张沛沛 杨德龙[1,2] DOU Jiaxin;TIAN Tian;WANG Peng;LIU Yuan;CHEN Tao;ZHANG Peipei;YANG Delong(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;State Key Laboratory of Aridland Crop Science,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]干旱生境作物学国家重点实验室,甘肃兰州730070

出　　处：《华北农学报》2023年第5期39-50,共12页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(32260520);甘肃省高等学校产业支撑计划项目(2022CYZC-44);中央引导地方科技发展资金项目(23ZYQA0322);甘肃省农业重点研发计划(21YF5NA089);甘肃省自然科学基金项目(21JR7RA828);甘肃省高等学校创新基金项目(2021B-125)。

摘　　要：蔗糖是小麦茎秆可溶性碳水化合物的主要运输形式,对小麦生长发育和籽粒灌浆起关键调控作用,属于典型的数量性状,遗传基础复杂。在全基因组水平上挖掘小麦茎秆蔗糖积累转运显著相关的SNP位点及候选基因,为相关基因克隆和分子标记辅助选择提供理论依据。在不同环境条件下,通过测定117份国内小麦品种开花期、灌浆期和成熟期茎秆蔗糖含量,计算蔗糖花前、花后转运率和对籽粒灌浆的贡献率等7个性状的表型,基于35K SNP芯片基因型分型,采用混合线性模型MLM(Q+K)进行全基因组关联分析(GWAS)及候选基因预测。在不同环境条件下,不同小麦品种茎秆蔗糖积累转运相关性状表型变异广泛,变异系数为9.53%~45.70%,广义遗传力为0.32~0.49。基因分型表明,SNP标记多态性信息含量(PIC)为0.10~0.38,群体结构分析将供试材料分为4个亚群。通过MLM(Q+K)模型检测到146个与茎秆蔗糖积累转运相关性状显著关联的SNP位点,其中3个位点均在2个环境中被检测到。对稳定显著关联的SNP位点上下游各200 kb物理位置范围内进行候选基因挖掘,筛选出13个与蔗糖积累转运相关性状有关的候选基因,其中TraesCS1B02G324100(核苷二磷酸糖转移酶基因)、TraesCS1B02G324200(纤维素合酶基因)、TraesCS1B02G324300(β-1,3-葡聚糖酶基因)与糖代谢相关。Sucrose is the main form of water-soluble carbohydrates transported in wheat stems,and plays a key role in the regulation of wheat growth and grain filling,which is a typical quantitative trait with a complex genetic basis.The SNP loci and candidate genes significantly associated with sucrose accumulation and translocation in wheat stems were explored at the genome-wide level to provide a theoretical basis for gene cloning and molecular marker-assisted selection.Seven traits of 117 domestic wheat varieties were measured,such as the sucrose content of flowering,filling,and maturity,the translocation rate and contribution rate to grain filling of sucrose at the pre-anthesis and post-anthesis,under different environmental conditions.Based on 35K SNP microarray genotyping,a mixed linear model MLM(Q+K)was used for genome-wide association study(GWAS)and candidate gene prediction.The overall phenotypic variation ranged from 9.53%to 45.70%with rich phenotypic diversity;the heritability of seven traits ranged from 0.32 to 0.49;the genotyping results showed that the polymorphism information content(PIC)of SNP markers ranged from 0.10 to 0.38;the population structure analysis divided the tested materials into four subgroups.The MLM(Q+K)model detected 146 significantly associated loci for traits related to sucrose accumulation and transport,among them,three SNP loci were detected repeatedly in two environments,and 13 candidate genes related to sucrose accumulation and transport related traits were screened based on the 200 kb physical location interval upstream and downstream of the 3 SNP loci that were stably and significantly associated.Among them,TraesCS1B02G324100(Nucleotide-diphospho-suger transferase gene),TraesCS1B02G324200(Cellulose synthase-like protein gene),and TraesCS1B02G324300(β-1,3-glucanase gene)were associated with sugar metabolism.

关 键 词：小麦 蔗糖 SNP 全基因组关联分析 候选基因 

分 类 号：S512.03[农业科学—作物学]