基于不同配体的NSE检测方法研究进展  

Advances in the detection methods for neuron-specific enolase based on different ligands

作　　者：梁展玮 彭涛 胡亚琳 屈子裕 路欣 焦学诗玛 王一楠 俞晓平 LIANG Zhanwei;PENG Tao;HU Yalin;QU Ziyu;LU Xin;JIAO Xueshima;WANG Yinan;YU Xiaoping(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;National Institute of Metrology,Beijing 100029,China;Changchun Institute of Metrology,Changchun 130012,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310018 [2]中国计量科学研究院,北京100029 [3]长春市计量检定测试技术研究院,吉林长春130012

出　　处：《中国计量大学学报》2023年第3期348-356,464,共10页Journal of China University of Metrology

基　　金：第七届青托工程项目(No.2021QNRC001)。

摘　　要：目的:通过综述神经特异性烯醇化酶(neuron-specific enolase,NSE)检测方法研究进展,为NSE灵敏准确定量检测方法开发提供有力解决方案。方法:结合文献和数据库搜索,总结NSE的结构特征与基本性质,系统介绍近年来基于不同配体的NSE检测方法与技术的研究进展。结果:作为目前最可靠的小细胞肺癌诊断标志物,NSE已被应用于开发多种检测方法与技术,并被应用于临床中肺癌的诊断、预后及疗效评估。结论:目前尚无统一的金标准方法来检测NSE,但已经开发出了多种基于不同配体的检测方法,它们可以用于恶性肿瘤等重大疾病的早期诊断、病情监控和预后评估,为临床提供技术支持。Aims:This paper aims to provide a powerful solution for the sensitive,accurate quantitation detection of neuron-specific enolase(NSE)by reviewing the research progress of the NSE detection methods.Methods:Combined with literature and database search,a variety of NSE detection methods and technologies based on different ligands developed in recent years were introduced.Results:As the most reliable diagnostic marker of small cell lung cancer at present,NSE has been applied to develop a variety of detection methods and technologies,and has been used in the clinical diagnosis,prognosis,and efficacy evaluation of lung cancer.Conclusions:There is no unified gold standard method for detecting NSE nowadays,but a variety of detection methods based on different ligands have been developed,which can be used for the early diagnosis,disease monitoring,and prognosis evaluation of major diseases such as malignant tumors,thus providing technical support for clinical use.

关 键 词：神经元特异性烯醇化酶 小细胞肺癌 肿瘤标志物 临床诊断 体外检测 

分 类 号：R730.43[医药卫生—肿瘤]