A comprehensive overview of cotton genomics,biotechnology and molecular biological studies  

作　　者：Xingpeng Wen Zhiwen Chen Zuoren Yang Maojun Wang Shuangxia Jin Guangda Wang Li Zhang Lingjian Wang Jianying Li Sumbul Saeed Shoupu He Zhi Wang Kun Wang Zhaosheng Kong Fuguang Li Xianlong Zhang Xiaoya Chen Yuxian Zhu 

机构地区：[1]Institute for Advanced Studies,Wuhan University,Wuhan 430072,China [2]College of Life Sciences,Wuhan University,Wuhan 430072,China [3]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,University of CAS,Chinese Academy of Sciences,Shanghai 200032,China [4]Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China [5]State Key Laboratory of Cotton Biology,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,China [6]Hubei Hongshan Laboratory,National Key Laboratory of Crop Genetic Improvement,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [7]State Key Laboratory of Plant Genomics,Institute of Microbiology,Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [8]Shanxi Agricultural University,Jinzhong 030801,China

出　　处：《Science China(Life Sciences)》2023年第10期2214-2256,共43页中国科学（生命科学英文版）

基　　金：the National Natural Science Foundation of China(32200286);the China Postdoctoral Science Foundation(2022TQ0240,2022M722470)。

摘　　要：Cotton is an irreplaceable economic crop currently domesticated in the human world for its extremely elongated fiber cells specialized in seed epidermis,which makes it of high research and application value.To date,numerous research on cotton has navigated various aspects,from multi-genome assembly,genome editing,mechanism of fiber development,metabolite biosynthesis,and analysis to genetic breeding.Genomic and 3D genomic studies reveal the origin of cotton species and the spatiotemporal asymmetric chromatin structure in fibers.Mature multiple genome editing systems,such as CRISPR/Cas9,Cas12(Cpf1)and cytidine base editing(CBE),have been widely used in the study of candidate genes affecting fiber development.Based on this,the cotton fiber cell development network has been preliminarily drawn.Among them,the MYB-b HLH-WDR(MBW)transcription factor complex and IAA and BR signaling pathway regulate the initiation;various plant hormones,including ethylene,mediated regulatory network and membrane protein overlap fine-regulate elongation.Multistage transcription factors targeting Ces A 4,7,and 8 specifically dominate the whole process of secondary cell wall thickening.And fluorescently labeled cytoskeletal proteins can observe real-time dynamic changes in fiber development.Furthermore,research on the synthesis of cotton secondary metabolite gossypol,resistance to diseases and insect pests,plant architecture regulation,and seed oil utilization are all conducive to finding more high-quality breeding-related genes and subsequently facilitating the cultivation of better cotton varieties.This review summarizes the paramount research achievements in cotton molecular biology over the last few decades from the above aspects,thereby enabling us to conduct a status review on the current studies of cotton and provide strong theoretical support for the future direction.

关 键 词：COTTON genome assembly genome editing fiber development GOSSYPOL plant architecture oil utilization genetic breeding 

分 类 号：S562[农业科学—作物学]