外周血白细胞介素-17、白细胞介素-8炎症因子在帕金森病发病机制中的研究  

Study on role of inflammatory factorsIL-17 and IL-8 in peripheral blood in pathogenesis of Parkinson's disease

作　　者：丁小川 李强[1] 时鹏[1] 吴梦琦 Ding Xiaochuan;Li Qiang;Shi Peng;Wu Mengqi(The First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui233000,China)

机构地区：[1]蚌埠医学院第一附属医院,安徽蚌埠233000

出　　处：《齐齐哈尔医学院学报》2023年第15期1418-1422,共5页Journal of Qiqihar Medical University

基　　金：安徽高校自然科学研究项目(KJ2021ZD0084)。

摘　　要：目的探讨外周血白介素-17(IL-17)、白介素-8(IL-8)炎症因子在帕金森病(PD)发病机制中的意义。方法选择2021年11月—2022年4月本院收治的38例PD患者作为实验组,收集同期体检中心健康志愿者38名作为对照组。将38例PD患者按病情进展分为早期12例(H&Y分期<2)、中期11例(2≤H&Y分期<3)、晚期15例(H&Y分期≥3);按UPDRS统一帕金森评定量表对38例PD患者进行分组,其中轻度7例(UPDRS评分≤50分)、中度21例(UPDRS评分≤100分)、重度10例(UPDRS评分>100分)。采集所有受试者外周血,采用生物素双抗体夹心酶联免疫吸附法检测血清中IL-17、IL-8含量,对外周血IL-17、IL-8与性别、年龄、患病年限、体重指数(BMI)、有无高血压病、有无糖尿病、甘油三酯、胆固醇、C反应蛋白(CRP)进行相关性分析。结果实验组外周血IL-17水平、BMI高于对照组,差异有统计学意义(t=6.68,P=0.001;t=2.84,P=0.006);实验组IL-8水平较对照组,差异无统计学意义(P>0.05)。根据H&Y评分,三组中IL-17存在统计学差异(F=78.00,P<0.001),其中晚期组IL-17水平最高,中期组水平高于早期组;三组中患病年限总体分布存在统计学差异(H=13.03,P=0.001),其中早期组与晚期组存在统计学差异(P=0.01),中期组与晚期组存在统计学差异(P=0.005)。根据UPDRS统一帕金森评定量表,三组中患病年限总体分布存在统计学差异(H=7.67,P=0.02),其中轻度组与中度组分布存在统计学差异(P=0.017),轻度组与重度组、中度组与重度组分布无统计学差异(P>0.05)。实验组患者外周血IL-17与H&Y分期呈正相关(r=0.934,P=0.001),患病年限与H&Y分期呈正相关(r=0.513,P=0.001),IL-17与患病年限之间呈正相关(r=0.523,P=0.001),有统计学意义。结论PD患者外周血IL-17水平高于健康人群,可能在PD发病机制中有一定作用。PD患者患病年限越长、症状越严重,IL-17水平越高。Objective To study the significance of inflammatory factorsinterleukin-17(IL-17)and interleukin-8(IL-8)peripheral blood in the pathogenesis of Parkinson's disease(PD).Methods From November 2021 to April 2022,38patients suffered from PD those were admitted to our hospital were selected as the experimentalgroup,and 38 healthy volunteers from the physical examination center during the same period were collected as the control group.38 PD patients were divided into three stages according to the progress of the disease:early stage group(12 cases,H&Y stage<2),medium stage group(11 cases,2≤H&Y<3),advanced stage group(15 cases,H&Y≥3).38 PD patients were divided into three groups according to UPDRS unified Parkinson's Rating Scale:mild group(7cases,UPDRS≤50),moderate group(21cases,UPDRS≤100),severe group(10 cases,UPDRS>100).Peripheral blood of all subjects were collected,and the levels of IL-17 and IL-8 in serum were detected by biotin double antibody sandwich enzyme-linked immunosorbent assay.The correlation between IL-17 and IL-8 in peripheral blood and gender,age,years of illness,body mass index(BMI),hypertension,diabetes,triglyceride,cholesterol and C-reactive protein(CRP)was analyzed.Results The levels of IL-17 in peripheral bloodand BMI of the experimental group were higher than those of the control group,and the differences were statistically significant(t=6.68,P=0.001;t=2.84,P=0.006).There was no significant difference in IL-8 level between the experimental group and the control group(P>0.05).According to H&Y score,there was statistical difference in IL-17 level among the three groups(F=78.00,P<0.001).The level of IL-17 in the late group was the highest,and the level of IL-17 in the intermediate group was higher than that in the early group.There were statistical differences in the overall distribution of disease duration among the three groups(H=13.03,P=0.001),among which there was statistical differences between the early group and the late group(P=0.01),and between the intermediate group and the late gr

关 键 词：帕金森病 白介素17 白介素8 炎症因子 

分 类 号：R742.5[医药卫生—神经病学与精神病学]