龙葵提取物对大肠杆菌、粪肠球菌及其生物膜形成的抑制作用  

Inhibitory Effect of Solanum nigrum L.Extracts on Escherichia coli and Enterococcus faecalis and Biofilm Formation

作　　者：郭航帆 王萍[1,2] 王颖[3] GUO Hangfan;WANG Ping;WANG Ying(School of Forestry,Northeast Forestry University,Harbin 150040,China;Key Laboratory of Forest Food Resources Utilization,Northeast Forestry University,Harbin 150040,China;Research Division of Heilongjiang Academy of Land Reclamation Sciences,Harbin 150038,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]东北林业大学森林食品资源利用重点实验室,哈尔滨150040 [3]黑龙江省农垦科学院科研处,哈尔滨150038

出　　处：《中国农业科技导报》2023年第10期152-164,共13页Journal of Agricultural Science and Technology

摘　　要：为探究龙葵提取物对大肠杆菌、粪肠球菌的抑菌作用及其对生物膜形成的影响,比较了黑果龙葵、黄果龙葵、红果龙葵果及其茎叶提取物的抑菌能力,并对黑果龙葵果提取工艺进行响应面优化;通过结晶紫染色法测定黑果龙葵果提取物抑制细菌生物膜形成能力,以疏水性、泳动性、自聚集性为指标,评价提取物对生物膜形成的抑制作用。结果表明,3种龙葵中黑果龙葵果提取物抑菌效果最好,抑制大肠杆菌生长最佳提取条件为提取温度28℃、乙醇含量44.5%、液料比5.1∶1(mL·g^(-1)),抑制粪肠球菌生长最佳提取条件为提取温度29.5℃,乙醇含量44.7%,液料比5.1∶1(mL·g^(-1)),此时黑果龙葵果提取物对大肠杆菌、粪肠球菌的抑菌率分别为81.44%±1.78%、90.63%±2.33%,最小抑菌质量浓度均为50 mg·mL^(-1)。30 mg·mL^(-1)黑果龙葵果提取物对大肠杆菌、粪肠球菌生物膜形成的抑制率分别为83.52%、85.13%,提取物抑制大肠杆菌生物膜形成与降低疏水性显著相关(P<0.05),抑制粪肠球菌生物膜形成与降低泳动性、自聚集性显著相关(P<0.05)。上述结果可为龙葵抑菌和生物膜抑制提供理论依据,为开发天然植物抗生素替代品提供参考。To investigate the antibacterial effect of Solanum nigrum L.on Escherichia coli and Enterococcus faecalis and its effect on biofilm formation,the antibacterial activities of extracts from Solanum nigrum L.,Solanum villosum Miller.,Solanum diphyllum L.fruit and their stems or leaves were compared,and the extracting conditions of Solanum nigrum L.fruit was optimized by response surface methodology.The effect of extracts from Solanum nigrum L.fruit on the formation of bacterial biofilm was determined by crystal violet staining,and the factors affecting the formation of antibiofilm were evaluated by swimming,auto-aggregation,and hydrophobicity.The results showed that the extracts from Solanum nigrum L.fruit had the best inhibition rate.The optimum extraction conditions for inhibiting the Escherichia coli were extraction temperature 28℃,ethanol content 44.5%,liquid-solid ratio 5.1∶1(mL·g^(-1));the optimum extraction conditions for inhibiting the Enterococcus faecalis were extraction temperature 29.5℃,ethanol content 44.7%,liquid-solid ratio 5.1∶1(mL·g^(-1)).Under these conditions,the extracts showed the best antibacterial effect on Escherichia coli and Enterococcus faecalis,the inhibition rate were 81.44%±1.78%and 90.63%±2.33%.The minimum inhibitory concentration of Escherichia coli and Enterococcus faecalis was 50 mg·mL^(-1).The inhibitory rates of 30 mg·mL^(-1) Solanum nigrum L.fruit extract on biofilm formation of Escherichia coli and Enterococcus faecalis were 83.52%and 85.13%,respectively.And the inhibition of Escherichia coli biofilm formation was related to the decrease of hydrophobicity(P<0.05),and the inhibition of Enterococcus faecalis biofilm formation was related to the decrease of swimming and auto-aggregation(P<0.05).These results provided a theoretical basis for the bacteria inhibition and biofilm inhibition of Solanum nigrum L.,and a reference for the development of natural plant antibiotic substitutes.

关 键 词：龙葵提取物 抑菌 理化性质 生物膜 

分 类 号：R285[医药卫生—中药学]