基于Nrf2和NLRP3探讨辣木籽提取物对慢性酒精性肝损伤小鼠肝脏的保护作用  

Protective Effect of Moringa oleifera Seed Extract in a Chronic Alcoholic Liver Injury Murine Model Based on NRF2 and NLRP3

作　　者：李晨雯 柏珺 赵力 杨思昱 罗雅俊 张青碧 LI Chenwen;BAI Jun;ZHAO Li;YANG Siyu;LUO Yajun;ZHANG Qingbi(Environmental Health Effects and Risk Assessment Key Laboratory of Luzhou,School of Public Health,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学公共卫生学院,环境健康效应及风险评估泸州市重点实验室,四川泸州646000

出　　处：《现代食品科技》2023年第10期51-58,共8页Modern Food Science and Technology

基　　金：国家级大学生创新创业训练计划项目(202010632056)。

摘　　要：该研究探讨了辣木籽提取物通过调控核转录NF-E2相关因子2(Nuclear Factor Crythroid-related Factor 2,Nrf2)和核苷酸结合寡聚化合物结构域样受体3(NACHT-LRR-PYD-containing Proteins 3 Inflammasome,NLRP3)通路减轻小鼠慢性酒精性肝损伤的作用机制。通过酒精液体饲料建立慢性酒精性肝损伤模型,干预组在酒精液体饲料喂养的同时用不同浓度辣木籽提取物灌胃,检测天冬氨酸氨基转移酶(Aspartate Aminotransferase,AST)、丙氨酸氨基转移酶(Alanine Aminotransferase,ALT)、白细胞介素-18(Interleukin-18,IL-18)、白细胞介素-1β(Interleukin-1β,IL-1β)、丙二醛(Malondialdehyde,MDA)、还原型谷胱甘肽(Glutathione,GSH)和超氧化物歧化酶(Superoxide Dismutase,SOD)含量,观察小鼠肝脏病理学改变,检测Nrf2、HO-1、NLRP3和Caspase-1的蛋白表达水平。结果表明,在辣木籽提取物的作用下,慢性酒精性肝损伤小鼠血清的AST和ALT最高分别下降至71.68 U/L、46.84 U/L(P<0.05),炎症因子IL-18及IL-1β含量下降;肝脏中GSH及SOD含量升高,MDA含量减少,Nrf2及HO-1表达升高,NLRP3及Caspase-1表达降低。提示辣木籽提取物通过Nrf2/HO-1和NLRP3/Caspase-1信号通路起到抗氧化及抑制炎症作用,从而减轻小鼠慢性酒精性肝损伤。The mechanism underlying the ability of Moringa oleifera seed extract to reduce chronic alcoholic liver injury in mice was anlayzed.The chronic alcoholic liver injury model was established by providing liquid alcohol feed to mice.The intervention group was also administered different concentrations of horseradish seed extract via oral gavage.Subsequently,aspartate aminotransferase(AST),alanine aminotransferase(ALT),interleukin-18(IL-18),interleukin-1β(IL-1β),malondialdehyde(MDA),reduced glutathione(GSH),and superoxide dismutase(SOD)contents were measured to assess the pathological changes in the mouse liver.Moreover,the expression of specific proteins,namely,nuclear factor crystalloid-related factor 2(NRF2),HO-1,nucleotide binding oligomeric domain like receptor 3(NLRP3),and caspase-1 were measured.The results showed that serum AST and ALT levels in the mice with chronic alcoholic liver injury decreased to 71.68 U/L and 46.84 U/L respectively(P<0.05)following the administration of M.oleifera seed extract.Following treatment,the abundance of inflammatory factors(IL-18 wand IL-1β)decreased,whereas the GSH and SOD levels increased in the liver.Moreover,MDA content and NLRP3 and caspase-1 abudance decreased,whereas the expression of NRF2 and HO-1 increased.These findings suggest that M.oleifera seed extract elicits antioxidant and anti-inflammatory effects through the NRF2/HO-1 and NLRP3/caspase-1 signaling pathways.

关 键 词：辣木籽 慢性酒精性肝损伤 氧化应激 炎症 

分 类 号：TS201.4[轻工技术与工程—食品科学]