榆黄菇多糖提取工艺优化及其免疫调节活性评价  被引量:4

Optimization of the Extraction of Polysaccharides from Pleurotus citrinopileatus and Evaluation of Their Immunomodulatory Activity

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作  者:文丁苑 梁双敏 国琦 宋晓晓 葛长荣[1] 肖智超 WEN Dingyuan;LIANG Shuangmin;GUO Qi;SONG Xiaoxiao;GE Changrong;XIAO Zhichao(Livestock Product Processing and Engineering Technology Research Center of Yunnan Province,Yunnan Agricultural University,Kunming 650201,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区:[1]云南农业大学云南省畜产品加工工程技术研究中心,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201 [3]云南农业大学动物科学技术学院,云南昆明650201

出  处:《现代食品科技》2023年第10期233-243,共11页Modern Food Science and Technology

基  金:云南省科技厅科技计划项目(202102AE090027);云南省博士后科研基金项目。

摘  要:以榆黄菇为研究对象,对榆黄菇多糖(PCP)提取工艺进行优化、纯化、测定单糖组成和分子量,再对其体外免疫活性进行评价。在单因素基础上通过响应面法确定榆黄菇多糖最优提取工艺为:提取温度59.81℃,提取时间2.40 h,液料比29.91 mL/g,在此条件下,预测得率为18.82%,实际得率为18.60%。经DEAE-Sepharose Fast Flow柱层析从榆黄菇多糖中分离纯化出3个多糖组分(PCP-1、PCP-2、PCP-3),采用离子色谱法和高效凝胶渗透色谱法分析多糖成分和分子量,得出PCP-1由半乳糖组成,分子量为1.90×10^(4)u;PCP-2由半乳糖和葡萄糖组成,摩尔比为4.36:5.64,分子量2.76×10^(4)u;PCP-3由岩藻糖、盐酸氨基葡萄糖、半乳糖、葡萄糖和葡萄糖醛酸组成,其摩尔比为0.07:0.11:0.51:7.46:1.85,分子量为4.81×10^(4)u。通过体外试验评估PCP对巨噬细胞RAW264.7免疫调节活性。结果表明,PCP-1、PCP-2和PCP-3多糖质量浓度在25~200μg/mL范围内对巨噬细胞RAW264.7无毒性并具有一定增殖作用、显著提高了NO释放量并增强了巨噬细胞RAW264.7的吞噬能力。当PCP-1、PCP-2和PCP-3多糖质量浓度为200μg/mL时,巨噬细胞RAW264.7 NO释放量达到最大值,分别为11.40、11.56、11.76μmoL/L。该研究结果可为榆黄菇的精深加工综合利用提供理论依据。The extraction of polysaccharides from Pleurotus citrinopileatus(PCP)was optimized,and the immunomodulatory effects of the purified polysaccharides were assessed.The optimal conditions to extract polysaccharides from P.citrinopileatus were determined using a single-factor response surface methodology and comprised an extraction temperature of 59.81℃,extraction time of 2.40 h,and liquid-solid ratio of 29.91 mL/g.Under these conditions,the predicted yield was 18.82%,and the actual yield was 18.60%.Three polysaccharide components(PCP-1,PCP-2,and PCP-3)were separated and purified from PCP using DEAE Sepharose Fast Flow column.The polysaccharide compositions and molecular weights were analyzed using ion chromatography and high-performance gel permeation chromatography.PCP-1 was found to be composed of galactose,with a molecular weight of 1.90×10^(4)u;PCP-2 was composed of galactose and glucose at a molar ratio of 4.36:5.64,with a molecular weight of 2.76×10^(4)u;PCP-3 was composed of fucose,glucosamine hydrochloride,galactose,glucose,and glucuronic acid at a molar ratio of 0.07:0.11:0.51:7.46:1.85,with a molecular weight of 4.81×10^(4)u.The immunomodulatory activity of PCP in macrophage RAW264.7 cultures was evaluated in vitro.PCP-1,PCP-2,and PCP-3 at 25~200 g/mL were non-toxic to RAW264.7 cells and enhanced their proliferation.Moreover,the polysaccharides substantially increased NO release and enhanced the phagocytic ability of RAW264.7 cells.Additionally,following treatment with PCP-1,PCP-2,or PCP-3 at 200μg/mL,NO production by RAW264.7 peaked at 11.40,11.56,and 11.76μmoL/L,respectively.

关 键 词:榆黄菇多糖 得率 单糖组成 免疫调节作用 

分 类 号:TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]

 

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